PURIFICATION AND PROPERTIES OF THE NITRILE HYDRATASE OF A NEW STRAIN OF RHODOCOCCUS SP

被引：6
|
作者：
KAAKEH, MR [1 ]
LEGRAS, JL [1 ]
DURAN, R [1 ]
CHION, CKNCK [1 ]
ARNAUD, A [1 ]
GALZY, P [1 ]
机构：
[1] ECOLE NATL SUPER AGRON,CHAIRE MICROBIOL,IND & GENET MICROORGAN,PL VIALA,F-34060 MONTPELLIER,FRANCE
来源：
ZENTRALBLATT FUR MIKROBIOLOGIE | 1991年 / 146卷 / 02期
关键词：
PURIFICATION; NITRILE HYDRATASE; RHODOCOCCUS;
D O I：
10.1016/S0232-4393(11)80286-6
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A strain of bacteria with nitrile hydratase activity was isolated from soil. Taxonomic studies indicated that the strain 7 belongs to the genus Rhodococcus. Its nitrile hydratase was purified and crystallized (from the crude extract). The enzyme has a molecular mass of about 278,000 Da. and consists of two kinds of subunits, with molecular masses of 26,000 Da. and 32,000 Da. The enzyme was active toward various aliphatic and aromatic nitriles.
引用
收藏
页码：89 / 98
页数：10
相关论文
共 50 条
  • [1] PURIFICATION AND CHARACTERIZATION OF NITRILE HYDRATASE OF A MUTANT STRAIN OF BREVIBACTERIUM SP
    MOREAU, JL
    AZZA, S
    ARNAUD, A
    GALZY, P
    JOURNAL OF BASIC MICROBIOLOGY, 1993, 33 (05) : 323 - 329
  • [2] Nitrile hydratase involved in aldoxime metabolism from Rhodococcus sp strain YH3-3 -: Purification and characterization
    Kato, Y
    Tsuda, T
    Asano, Y
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 263 (03): : 662 - 670
  • [3] ISOLATION AND CHARACTERIZATION OF A NITRILE HYDRATASE FROM A RHODOCOCCUS SP
    HJORT, CM
    GODTFREDSEN, SE
    EMBORG, C
    JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1990, 48 (02) : 217 - 226
  • [4] Purification and characterization of a novel nitrile hydratase from Rhodococcus sp. RHA1
    Okamoto, Sachi
    Eltis, Lindsay D.
    MOLECULAR MICROBIOLOGY, 2007, 65 (03) : 828 - 838
  • [5] NITRILE HYDRATASE OF RHODOCOCCUS SP N-774 - PURIFICATION AND AMINO-ACID SEQUENCES
    ENDO, T
    WATANABE, I
    FEBS LETTERS, 1989, 243 (01) : 61 - 64
  • [6] Purification and characterization of Rhodococcus boritolerans FW815 nitrile hydratase
    Zheng, Y.-G. (zhengyg@zjut.edu.cn), 1600, Zhejiang University (27):
  • [7] Effects of nitriles and amides on the growth and nitrile hydratase activity of the Rhodococcus sp strain gt1
    Maksimov, AY
    Kuznetsova, MV
    Ovechkina, GV
    Kozlov, SV
    Maksimova, YG
    Demakov, VA
    APPLIED BIOCHEMISTRY AND MICROBIOLOGY, 2003, 39 (01) : 55 - 59
  • [8] PHOTOSENSITIVE PHENOMENA OF NITRILE HYDRATASE OF RHODOCOCCUS SP N-771
    NAGAMUNE, T
    KURATA, H
    HIRATA, M
    HONDA, J
    HIRATA, A
    ENDO, I
    PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1990, 51 (01) : 87 - 90
  • [9] Effects of Nitriles and Amides on the Growth and Nitrile Hydratase Activity of the Rhodococcus sp. Strain gt1
    A. Yu. Maksimov
    M. V. Kuznetsova
    G. V. Ovechkina
    S. V. Kozlov
    Yu. G. Maksimova
    V. A. Demakov
    Applied Biochemistry and Microbiology, 2003, 39 : 55 - 59
  • [10] Purification and characterization of nitrile hydratase from Rhodococcus rhodochrous M8
    Sinolitsky, MK
    Rogacheva, SM
    Poltavskaya, SV
    Sintin, AA
    Voronin, SP
    ENVIRONMENTAL BIOTECHNOLOGY: PRINCIPLES AND APPLICATIONS, 1996, : 96 - 104
12345