PURIFICATION AND PROPERTIES OF THE NITRILE HYDRATASE OF A NEW STRAIN OF RHODOCOCCUS SP

被引：6
|
作者：
KAAKEH, MR [1 ]
LEGRAS, JL [1 ]
DURAN, R [1 ]
CHION, CKNCK [1 ]
ARNAUD, A [1 ]
GALZY, P [1 ]
机构：
[1] ECOLE NATL SUPER AGRON,CHAIRE MICROBIOL,IND & GENET MICROORGAN,PL VIALA,F-34060 MONTPELLIER,FRANCE
来源：
ZENTRALBLATT FUR MIKROBIOLOGIE | 1991年 / 146卷 / 02期
关键词：
PURIFICATION; NITRILE HYDRATASE; RHODOCOCCUS;
D O I：
10.1016/S0232-4393(11)80286-6
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A strain of bacteria with nitrile hydratase activity was isolated from soil. Taxonomic studies indicated that the strain 7 belongs to the genus Rhodococcus. Its nitrile hydratase was purified and crystallized (from the crude extract). The enzyme has a molecular mass of about 278,000 Da. and consists of two kinds of subunits, with molecular masses of 26,000 Da. and 32,000 Da. The enzyme was active toward various aliphatic and aromatic nitriles.
引用
收藏
页码：89 / 98
页数：10
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