Effects of Nitriles and Amides on the Growth and Nitrile Hydratase Activity of the Rhodococcus sp. Strain gt1

被引：0
|
作者：
A. Yu. Maksimov
M. V. Kuznetsova
G. V. Ovechkina
S. V. Kozlov
Yu. G. Maksimova
V. A. Demakov
机构：
[1] Institute of Ecology and Genetics of Microorganisms,Ural Division, Russian Academy of Sciences
来源：
Applied Biochemistry and Microbiology | 2003年 / 39卷
关键词：
Nitrogen; Glucose; Ammonium; Amide; Nitrile;
D O I：
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中图分类号：
学科分类号：
摘要：
Effects of some nitriles and amides, as well as glucose and ammonium, on the growth and the nitrile hydratase (EC 4.2.1.84) activity of the Rhodococcus sp. strain gt1 isolated from soil were studied. The activity of nitrile hydratase mainly depended on the carbon and nitrogen supply to cells. The activity of nitrile hydratase was high in the presence of glucose and ammonium at medium concentrations and decreased at concentrations of glucose of more than 0.3%. Saturated unsubstituted aliphatic nitriles and amides were found to be a good source of nitrogen and carbon. However, the presence of nitriles and amides in the medium was not absolutely necessary for the expression of the activity of nitrile hydratase of the Rhodococcus sp. strain gt1.
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页码：55 / 59
页数：4
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