Purification and characterization of nitrile hydratase from Rhodococcus rhodochrous M8

被引：0
|
作者：
Sinolitsky, MK
Rogacheva, SM
Poltavskaya, SV
Sintin, AA
Voronin, SP
机构：
来源：
ENVIRONMENTAL BIOTECHNOLOGY: PRINCIPLES AND APPLICATIONS | 1996年
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中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The nitrile hydratase of Rhodococcus rhodochrous M8 has been purified and the enzyme properties studied. The molecular mass of the enzyme was 510 kDa. The nitrile hydratase was composed of two different types of subunits with molecular masses 23.5 kDa and 28 kDa. Optimal temperature and pH values for the enzyme activity and stability were: 20-30 degrees C, pH 7.0-7.2. The nitrile hydratase activity was stabilized by butanoic acid. The enzyme exhibited high activity towards aliphatic substrates. The aminoterminal amino acid sequence was obtained.
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页码：96 / 104
页数：9
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