PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study

被引：0
|
作者：
Feng Wang
Hebai Shen
Jie Feng
Haifeng Yang
机构：
[1] College of Life and Environment Science,
[2] Shanghai Normal University,undefined
来源：
Microchimica Acta | 2006年 / 153卷
关键词：
Key words: Peptide nucleic acid (PNA); magnetic nanoparticles (MNPs); PNA-modified MNPs (PMNPs); surface-enhanced Raman scattering (SERS).;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A 4-pyridyldithiol-derivatized peptide nucleic acid (PNA), which was designed to recognize a specific gene, was attached to 3-mercapropropyloxysilane coated magnetic nanoparticles (MNPs) via a thiol-disulfide exchange reaction. Subsequently, PNA-functionalized magnetic nanoparticles (PMNPs) were prepared. Sequentially the PMNPs were challenged with non-complementary and perfect-match DNA targets. The entire procedure was monitored using surface-enhanced Raman scattering (SERS). The results showed that the PMNPs were able to efficiently hybridize with the perfect-match ssDNA target and showed no affinity towards non-complementary DNA. This approach may provide a means of direct and label-free gene analysis.
引用
收藏
页码：15 / 20
页数：5
相关论文
共 50 条
  • [21] PCR-SSO hybridization in microplate method using single-stranded DNA dissociated by alkaline-magnetic beads
    Kobayashi, K
    Hata, Y
    Iwasaki, M
    Atoh, M
    Konoeda, Y
    Yagita, A
    Sekiguchi, S
    HUMAN IMMUNOLOGY, 1996, 47 (1-2) : O210 - O210
  • [22] PCR-SSO hybridization in microplate method using single-stranded DNA dissociated by alkaline-magnetic beads
    Kobayashi, K
    Hata, Y
    Iwasaki, M
    Atoh, M
    Suzuki, H
    Sekiguchi, S
    HLA - GENETIC DIVERSITY OF HLA FUNCTIONAL AND MEDICAL IMPLICATION, PROCEEDINGS OF THE TWELFTH INTERNATIONAL HISTOCOMPATIBILITY WORKSHOP AND CONFERENCE (12TH IHWC), VOL II: CONFERENCE, 1997, : 326 - 329
  • [23] On-site detection of Phytophthora spp.—single-stranded target DNA as the limiting factor to improve on-chip hybridization
    Lydia Schwenkbier
    Stephan König
    Stefan Wagner
    Sibyll Pollok
    Jörg Weber
    Martin Hentschel
    Jürgen Popp
    Sabine Werres
    Karina Weber
    Microchimica Acta, 2014, 181 : 1669 - 1679
  • [24] Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA
    Takahashi, Hiromi
    Yasui, Takao
    Kashida, Hiromu
    Makino, Koki
    Shinjo, Keiko
    Liu, Quanli
    Shimada, Taisuke
    Rahong, Sakon
    Kaji, Noritada
    Asanuma, Hiroyuki
    Baba, Yoshinobu
    NANOTECHNOLOGY, 2021, 32 (25)
  • [25] Single-stranded DNA adsorption on chiral molecule coated Au surface: a molecular dynamics study
    Liang, Haiqing
    Li, Zhenyu
    Yang, Jinlong
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2010, 12 (17) : 4431 - 4434
  • [26] Sensitive and Label-Free SERS Detection of Single-Stranded DNA Assisted by Silver Nanoparticles and Gold-Coated Magnetic Nanoparticles
    Yu, Zhao
    Grasso, Michael F.
    Cui, Xiaoyu
    Silva, Rebecca Nogueira
    Zhang, Peng
    ACS APPLIED BIO MATERIALS, 2020, 3 (05): : 2626 - 2632
  • [27] DNA reorientation on Au nanoparticles: label-free detection of hybridization by surface enhanced Raman spectroscopy
    Papadopoulou, Evanthia
    Bell, Steven E. J.
    CHEMICAL COMMUNICATIONS, 2011, 47 (39) : 10966 - 10968
  • [28] Covalentimmobilization of oligoDNA on the surface of magnetic nanoparticles and surface-enhanced Raman scattering study
    SHEN Hebai1
    2. State Key Laboratory of Chemical & Biological Sensor and Metric
    3. Department of Physics
    ChineseScienceBulletin, 2003, (24) : 2698 - 2702
  • [29] Assembly of Single-Stranded DNA Onto HOPG Surface at Different Temperature: Atomic Force Microscopy Study
    Liu, Zhiguo
    Zhao, Lin
    Zhou, Zhen
    Sun, Tongze
    Zu, Yuangang
    SCANNING, 2012, 34 (05) : 302 - 308
  • [30] Surface-enhanced Raman scattering of DNA molecules on silver electrodes modified with silver nanoparticles
    Li, Jie
    VIBRATIONAL SPECTROSCOPY, 2022, 123
12345