PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study

被引：0
|
作者：
Feng Wang
Hebai Shen
Jie Feng
Haifeng Yang
机构：
[1] College of Life and Environment Science,
[2] Shanghai Normal University,undefined
来源：
Microchimica Acta | 2006年 / 153卷
关键词：
Key words: Peptide nucleic acid (PNA); magnetic nanoparticles (MNPs); PNA-modified MNPs (PMNPs); surface-enhanced Raman scattering (SERS).;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A 4-pyridyldithiol-derivatized peptide nucleic acid (PNA), which was designed to recognize a specific gene, was attached to 3-mercapropropyloxysilane coated magnetic nanoparticles (MNPs) via a thiol-disulfide exchange reaction. Subsequently, PNA-functionalized magnetic nanoparticles (PMNPs) were prepared. Sequentially the PMNPs were challenged with non-complementary and perfect-match DNA targets. The entire procedure was monitored using surface-enhanced Raman scattering (SERS). The results showed that the PMNPs were able to efficiently hybridize with the perfect-match ssDNA target and showed no affinity towards non-complementary DNA. This approach may provide a means of direct and label-free gene analysis.
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页码：15 / 20
页数：5
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