PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study

被引：0
|
作者：
Feng Wang
Hebai Shen
Jie Feng
Haifeng Yang
机构：
[1] College of Life and Environment Science,
[2] Shanghai Normal University,undefined
来源：
Microchimica Acta | 2006年 / 153卷
关键词：
Key words: Peptide nucleic acid (PNA); magnetic nanoparticles (MNPs); PNA-modified MNPs (PMNPs); surface-enhanced Raman scattering (SERS).;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A 4-pyridyldithiol-derivatized peptide nucleic acid (PNA), which was designed to recognize a specific gene, was attached to 3-mercapropropyloxysilane coated magnetic nanoparticles (MNPs) via a thiol-disulfide exchange reaction. Subsequently, PNA-functionalized magnetic nanoparticles (PMNPs) were prepared. Sequentially the PMNPs were challenged with non-complementary and perfect-match DNA targets. The entire procedure was monitored using surface-enhanced Raman scattering (SERS). The results showed that the PMNPs were able to efficiently hybridize with the perfect-match ssDNA target and showed no affinity towards non-complementary DNA. This approach may provide a means of direct and label-free gene analysis.
引用
收藏
页码：15 / 20
页数：5
相关论文
共 50 条
  • [41] Combined use of vancomycin-modified Ag-coated magnetic nanoparticles and secondary enhanced nanoparticles for rapid surface-enhanced Raman scattering detection of bacteria
    Wang, Chongwen
    Gu, Bing
    Liu, Qiqi
    Pang, Yuanfeng
    Xiao, Rui
    Wang, Shengqi
    INTERNATIONAL JOURNAL OF NANOMEDICINE, 2018, 13 : 1159 - 1178
  • [42] Adsorption of polyethyleneimine on silver nanoparticles and its interaction with a plasmid DNA:: A surface-enhanced Raman scattering study
    Sanchez-Cortes, S
    Berenguel, RM
    Madejón, A
    Pérez-Méndez, M
    BIOMACROMOLECULES, 2002, 3 (04) : 655 - 660
  • [43] Method for effective immobilization of Ag nanoparticles/graphene oxide composites on single-stranded DNA modified gold electrode for enzymeless H2O2 detection
    Lu, Wenbo
    Chang, Guohui
    Luo, Yonglan
    Liao, Fang
    Sun, Xuping
    JOURNAL OF MATERIALS SCIENCE, 2011, 46 (15) : 5260 - 5266
  • [44] Method for effective immobilization of Ag nanoparticles/graphene oxide composites on single-stranded DNA modified gold electrode for enzymeless H2O2 detection
    Wenbo Lu
    Guohui Chang
    Yonglan Luo
    Fang Liao
    Xuping Sun
    Journal of Materials Science, 2011, 46 : 5260 - 5266
  • [45] Polarization-based surface enhanced Raman scattering from single colloidal DNA decorated with 3 nm silicon nanoparticles
    Mantey, Kevin
    Quagliano, Lucia
    Rezk, Ayman
    Palleschi, Simonetta
    Abuhassan, Laila
    Nayfeh, Ammar
    Bahceci, Ersin
    Nayfeh, Munir H.
    AIP ADVANCES, 2021, 11 (10)
  • [46] Label-free detection of DNA methylation by surface-enhanced Raman spectroscopy using zirconium-modified silver nanoparticles
    Zhang, Yuan
    Zhan, De-Sheng
    Xu, Xiao-Ying
    Zhang, Zhe
    Hafez, Mahmoud Elsayed
    He, Yue
    Li, Yang
    Li, Da-Wei
    TALANTA, 2023, 253
  • [47] Surface-enhanced Raman scattering for HSP 70A mRNA detection in live cells using silica nanoparticles and DNA-modified gold nanoparticles
    Cho, Ju Eun
    Lim, Dong-Kwon
    JOURNAL OF MATERIALS CHEMISTRY B, 2025, 13 (02) : 562 - 567
  • [48] Target induced aggregation of modified Au@Ag nanoparticles for surface enhanced Raman scattering and its ultrasensitive detection of arsenic(III) in aqueous solution
    Yang, Bo
    Chen, Xiaochun
    Liu, Renyong
    Liu, Bianhua
    Jiang, Changlong
    RSC ADVANCES, 2015, 5 (95): : 77755 - 77759
  • [49] Improving surface-enhanced Raman scattering performance of gold-modified magnetic nanoparticles by using nickel-phosphorus film on polydimethylsiloxane
    Adamo, Cristina B.
    Poppi, Ronei J.
    de Jesus, Dosil P.
    MICROCHEMICAL JOURNAL, 2021, 160
  • [50] Surface-Enhanced Raman Scattering Detection of DNA Derived from the West Nile Virus Genome Using Magnetic Capture of Raman-Active Gold Nanoparticles
    Zhang, Hao
    Harpster, Mark H.
    Park, Hee Joon
    Johnson, Patrick A.
    ANALYTICAL CHEMISTRY, 2011, 83 (01) : 254 - 260
12345