The rapid evaluation of bacterial growth in blood cultures by selected ion flow tube-mass spectrometry (SIFT-MS) and comparison with the BacT/ALERT automated blood culture system

被引：57
|
作者：
Scotter, J. M.
Allardyce, R. A.
Langford, Vs.
Hill, A.
Murdoch, D. R.
机构：
[1] Syft Technol Ltd, Med Applicat, Christchurch, New Zealand
[2] Univ Otago, Christchurch Sch Med & Hlth Sci, Dunedin, New Zealand
[3] Canterbury Hlth Labs, Microbiol Unit, Christchurch, New Zealand
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 65卷 / 03期
关键词：
blood cultures; selected ion flow tube-mass spectrometry; BacT/ALERT;
D O I：
10.1016/j.mimet.2005.09.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms. (c) 2005 Elsevier B.V. All rights reserved.
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页码：628 / 631
页数：4
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