The rapid evaluation of bacterial growth in blood cultures by selected ion flow tube-mass spectrometry (SIFT-MS) and comparison with the BacT/ALERT automated blood culture system

被引：57

作者：

Scotter, J. M.

Allardyce, R. A.

Langford, Vs.

Hill, A.

Murdoch, D. R.

机构：

[1] Syft Technol Ltd, Med Applicat, Christchurch, New Zealand

[2] Univ Otago, Christchurch Sch Med & Hlth Sci, Dunedin, New Zealand

[3] Canterbury Hlth Labs, Microbiol Unit, Christchurch, New Zealand

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 65卷 / 03期

关键词：

blood cultures; selected ion flow tube-mass spectrometry; BacT/ALERT;

D O I：

10.1016/j.mimet.2005.09.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We compared the performance of the BacT/ALERT automated blood culture system with real-time, quantitative volatile organic compound (VOC) detection by selected ion flow tube-mass spectrometry (SIFT-MS). Blood samples from healthy donors were artificially infected with 5 or 100 CFU of organisms commonly causing bacteremia. Positive results by SIFT-MS analysis of headspace gases were recorded for 53/60 (88.3%) bottles at 8h, and 58/60 (96.6%) bottles at 24 h. We conclude that SIFT-MS is a sensitive method for the detection of microbial VOCs. Furthermore, profiles of the VOCs detected may allow simultaneous identification of infecting organisms. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：628 / 631

页数：4

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