Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media

被引：189

作者：

Kamiya, Noriho ^{[1
,2
]}

Matsushita, Yuichi ^{[1
]}

Hanaki, Misa ^{[1
]}

Nakashima, Kazunori ^{[1
]}

Narita, Mamiko ^{[3
]}

Goto, Masahiro ^{[1
,2
]}

Takahashi, Haruo ^{[3
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8190395, Japan

[2] Kyushu Univ, Ctr Future Chem, Fukuoka 8190395, Japan

[3] Toyota Cent Res & Dev Labs Inc, Biotechnol Lab, Aichi 48011, Japan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 06期

关键词：

biorefinery; cellulase; cellulose; ethanol; ionic liquid;

D O I：

10.1007/s10529-008-9638-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzymatic saccharification of a model cellulosic substrate, Avicel PH- 101, using an ionic liquid ( IL), 1- ethyl- 3- methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer ( pH 5.0), cellulase was directly added to the aqueous- IL mixture at 40 degrees C. When the volume of IL to water was greater than 3: 2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1: 4 ( v/ v) resulted in over 70% of the starting amount of cellulose ( 10 mg/ ml) being converted to glucose and cellobiose.

引用

下载

页码：1037 / 1040

页数：4

共 50 条

[21] Enzymatic saccharification by ionic liquid pretreatment for biofuels production
Mazzotta, Michael
Ellison, L. Hope
Morton, Laurel A.
Brock, Martin
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2011, 241
[22] Extraction of lignins from aqueous-ionic liquid mixtures by organic solvents
Xin, Qin
Pfeiffer, Katie
Prausnitz, John M.
Clark, Douglas S.
Blanch, Harvey W.
BIOTECHNOLOGY AND BIOENGINEERING, 2012, 109 (02) : 346 - 352
[23] Carbon dioxide reduction in biphasic aqueous-ionic liquid systems by pressurized hydrogen
Ohlin, CA
Laurenczy, G
HIGH PRESSURE RESEARCH, 2003, 23 (03) : 239 - 242
[24] SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CELLULOSE TO ETHANOL BY IONIC LIQUID PRETREATMENT
Liu, Zhen
Zhao, Dianjia
Qin, Wei
CELLULOSE CHEMISTRY AND TECHNOLOGY, 2015, 49 (02): : 175 - 179
[25] Modulating the Solubilities of Ionic Liquid Components in Aqueous-Ionic Liquid Biphasic Systems: A Q-NMR Investigation
Atanassova, Maria
Mazan, Valerie
Billard, Isabelle
CHEMPHYSCHEM, 2015, 16 (08) : 1703 - 1711
[26] SIMULTANEOUS SACCHARIFICATION AND FERMENTATION OF CELLULOSE - EFFECT OF ETHANOL ON ENZYMATIC SACCHARIFICATION OF CELLULOSE
OOSHIMA, H
ISHITANI, Y
HARANO, Y
BIOTECHNOLOGY AND BIOENGINEERING, 1985, 27 (04) : 389 - 397
[27] Simultaneous saccharification and fermentation of cellulose: Effect of ethanol on enzymatic saccharification of cellulose
Ooshima, H.
Ishitani, Y.
Harano, Y.
1600, (27):
[28] Enzymatic degradation of cellulose promoted by ionic liquid
Xu, Cuizhi
Li, Lu
Liu, Shiwei
Yu, Shitao
Liu, Fusheng
Xie, Congxia
Gaofenzi Cailiao Kexue Yu Gongcheng/Polymeric Materials Science and Engineering, 2011, 27 (05): : 85 - 88
[29] Enzymatic hydrolysis of cellulose in ionic liquid solutions
Wahlstrom, Ronny
Suurnakki, Anna
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
[30] Enzymatic in situ saccharification of chestnut shell with high ionic liquid-tolerant cellulases from Galactomyces sp CCZU11-1 in a biocompatible ionic liquid-cellulase media
He, Yu-Cai
Liu, Feng
Gong, Lei
Di, Jun-Hua
Ding, Yun
Ma, Cui-Luan
Zhang, Dan-Ping
Tao, Zhi-Cheng
Wang, Cheng
Yang, Bin
BIORESOURCE TECHNOLOGY, 2016, 201 : 133 - 139

← 1 2 3 4 5 →