Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media

被引:189
|
作者
Kamiya, Noriho [1 ,2 ]
Matsushita, Yuichi [1 ]
Hanaki, Misa [1 ]
Nakashima, Kazunori [1 ]
Narita, Mamiko [3 ]
Goto, Masahiro [1 ,2 ]
Takahashi, Haruo [3 ]
机构
[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8190395, Japan
[2] Kyushu Univ, Ctr Future Chem, Fukuoka 8190395, Japan
[3] Toyota Cent Res & Dev Labs Inc, Biotechnol Lab, Aichi 48011, Japan
关键词
biorefinery; cellulase; cellulose; ethanol; ionic liquid;
D O I
10.1007/s10529-008-9638-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The enzymatic saccharification of a model cellulosic substrate, Avicel PH- 101, using an ionic liquid ( IL), 1- ethyl- 3- methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer ( pH 5.0), cellulase was directly added to the aqueous- IL mixture at 40 degrees C. When the volume of IL to water was greater than 3: 2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1: 4 ( v/ v) resulted in over 70% of the starting amount of cellulose ( 10 mg/ ml) being converted to glucose and cellobiose.
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页码:1037 / 1040
页数:4
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