Enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media

被引：189

作者：

Kamiya, Noriho ^{[1
,2
]}

Matsushita, Yuichi ^{[1
]}

Hanaki, Misa ^{[1
]}

Nakashima, Kazunori ^{[1
]}

Narita, Mamiko ^{[3
]}

Goto, Masahiro ^{[1
,2
]}

Takahashi, Haruo ^{[3
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8190395, Japan

[2] Kyushu Univ, Ctr Future Chem, Fukuoka 8190395, Japan

[3] Toyota Cent Res & Dev Labs Inc, Biotechnol Lab, Aichi 48011, Japan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 06期

关键词：

biorefinery; cellulase; cellulose; ethanol; ionic liquid;

D O I：

10.1007/s10529-008-9638-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzymatic saccharification of a model cellulosic substrate, Avicel PH- 101, using an ionic liquid ( IL), 1- ethyl- 3- methylimidazolium diethylphosphate, was explored. After mixing the IL solution of cellulose with different volumes of 10 mM citrate buffer ( pH 5.0), cellulase was directly added to the aqueous- IL mixture at 40 degrees C. When the volume of IL to water was greater than 3: 2, little cellulase activity was observed. However, decreasing the volume ratio markedly enhanced enzymatic activity: an IL to water ratio of 1: 4 ( v/ v) resulted in over 70% of the starting amount of cellulose ( 10 mg/ ml) being converted to glucose and cellobiose.

引用

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页码：1037 / 1040

页数：4

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