Comparison of microarray and RNA-Seq analysis of mRNA expression in dermal mesenchymal stem cells

被引：39

作者：

Li, Junqin ^{[1
]}

Hou, Ruixia ^{[1
]}

Niu, Xuping ^{[1
]}

Liu, Ruifeng ^{[1
]}

Wang, Qiang ^{[1
]}

Wang, Chunfang ^{[2
]}

Li, Xinhua ^{[1
]}

Hao, Zhongping ^{[3
]}

Yin, Guohua ^{[1
]}

Zhang, Kaiming ^{[1
]}

机构：

[1] Taiyuan City Ctr Hosp, Inst Dermatol, Taiyuan 030009, Shanxi Province, Peoples R China

[2] Shanxi Med Univ, Lab Anim Ctr, Taiyuan 030001, Shanxi Province, Peoples R China

[3] Gen Hosp TISCO, Dept Dermatol, Taiyuan 030003, Shanxi Province, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2016年 / 38卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Dermal mesenchymal stem cells; Differential gene expression; Mesenchymal stem cells; Microarray; RNA sequencing; PSORIASIS; SKIN; TRANSCRIPTOME; GENES;

D O I：

10.1007/s10529-015-1963-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We characterized mRNA expression profiles in normal and psoriatic human dermal mesenchymal stem cells (DMSCs) to provide a reference for future investigation of differential gene expression in DMSCs. Microarray and RNA sequencing (RNA-Seq) analyses both identified 23 differentially expressed genes using both platforms. The results showed comparable upregulation or downregulation for 14/23 genes using either platform and a 100 % coincidence rate was found by real-time PCR. For all of the differentially expressed genes that were verified by real-time PCR, the coincidence rate for RNA-Seq and real-time PCR was significantly higher than that for microarray analysis and real-time PCR (83.3 vs. 37.5 %, P < 0.0001). Furthermore, RNA-Seq revealed the presence of over 2300 novel transcription tags. Relative to microarray analysis, RNA-Seq is more accurate in identifying differentially expressed genes in DMSCs.

引用

页码：33 / 41

页数：9

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