Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system

被引：0
|
作者：
Yi, Tong [1 ]
Liu, Jingjing [1 ]
Zhang, Siyao [1 ]
He, Yihua [1 ,2 ]
Han, Jiancheng [1 ,2 ]
机构：
[1] Capital Med Univ, Beijing Anzhen Hosp, Dept Ultrasound, Beijing Key Lab Maternal Fetus Med Fetal Heart Dis, Beijing, Peoples R China
[2] Beijing Anzhen Hosp, Dept Ultrasound, Beijing Key Lab Maternal Fetus Med Fetal Heart Dis, 2 Anzhen Rd, Beijing 100029, Peoples R China
来源：
STEM CELL RESEARCH | 2023年 / 67卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2023.103034
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The tissue inhibitors of metalloproteinases 3 (TIMP3) play an essential role in the tumorigenesis of human pancreatic endocrine tumors and Sorsby fundus dystrophy. To further investigate the significance of TIMP3 in disease, we used CRISPR/Cas9 to create a TIMP3 knock out human embryonic stem cell line (WAe009-A-89) that can differentiate into any desired cell type. Our results show that the WAe009-A-89 cell line retains the typical colony form and normal karyotype of stem cells. The cells strongly expressed pluripotency markers and could differentiate into tissues of all three germ layers in vivo. This cell line allowed exploring the role of the TIMP3 gene in related diseases.
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页数：4
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