Generation of PARP1 gene knockout human embryonic stem cell line using CRISPR/Cas9

被引：3
|
作者：
Li, Yuhang [1 ,2 ]
Li, Jinbing [1 ,2 ]
Zhou, Tiancheng [1 ]
Pan, Guangjin [1 ]
Huang, Ke [1 ,3 ]
机构：
[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, CAS Key Lab Regenerat Biol, Guangzhou 510530, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Sun Yat Sen Univ, Affiliated Hosp 7, Shenzhen 518107, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 53卷
关键词：
D O I：
10.1016/j.scr.2021.102288
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
PARP1 encodes a chromatin-associated enzyme which responsible for post-translational poly(ADP-ribosyl)ation modification (Hsieh et al., 2017). It plays an important role in nucleotide excision repair, non-homologous end joining, DNA mismatch repair and many other DNA repair process. Also, PARP1 participates in inflammation and aging. However, its role in human embryonic stem cell biology has not been fully resolved. To clarify the function of PARP1 in human embryonic stem cells, we reported a PARP1 knockout human embryonic stem cell line, generated by CRISPR/Cas9 mediated gene targeting. This cell line shows normal karyotype, pluripotent stem cell marker expression and differentiation potential in vitro.
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页数：5
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