Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR/Cas9 editing

被引：6
|
作者：
Xie, Liangqun [1 ]
Huang, Jingrui [1 ]
Li, Xun [1 ]
Dai, Lei [1 ]
Lin, Xinxiu [1 ]
Zhang, Jiejie [1 ]
Luo, Jiefeng [1 ]
Zhang, Weishe [1 ,2 ]
机构：
[1] Cent South Univ, Dept Obstet, Xiangya Hosp, 87 Xiangya Rd, Changsha 410008, Peoples R China
[2] Hunan Engn Res Ctr Early Life Dev & Dis Prevent, 87 Xiangya Rd, Changsha 410008, Peoples R China
来源：
STEM CELL RESEARCH | 2019年 / 41卷
关键词：
D O I：
10.1016/j.scr.2019.101610
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme in the HDAC family. The HDAC6 has been shown to play important roles in several biological processes. Meanwhile, it is also an attractive therapeutic target for a variety of diseases. However, the mechanism of HDAC6 function is not fully understood yet, and it is still lacking highly specific targeted drugs. Here, we generated a homozygous HDAC6 knockout human embryonic stem cell (hESC) line, WAe009-A-21 by the CRISPR/Cas9-based gene editing method. The WAe009-A-21 cell line does not express HDAC6 protein, while maintaining normal 46, XX karyotype, pluripotency, and trilineage differentiation potential.
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页数：4
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