Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system

被引：0
|
作者：
Zhang, Wang [1 ]
Li, Yuming [3 ]
Wang, Li [1 ]
Xin, Tao [3 ]
Zhang, Canwei [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510623, Peoples R China
[2] Shandong First Med Univ & Shandong Prov Qianfosha, Affiliated Hosp 1, Dept Ophthalmol, Jinan 250014, Peoples R China
[3] Shandong First Med Univ & Shandong Prov Qianfosha, Affiliated Hosp 1, Dept Neurosurg, Shandong Med & Hlth Key Lab Neurosurg, Jinan 250014, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 57卷
关键词：
D O I：
10.1016/j.scr.2021.102611
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Dickkopf-2 (DKK2) is an antagonist of canonical Wnt signaling, which is involved in various biological processes of development, such as epidermal appendage formation and eye development. To identify underlying effects of DKK2 during embryonic development, we generated a DKK2 homozygous knockout human embryonic stem cell (hESC) line through the CRISPR/Cas9 genome-editing technology. This cell line, which maintains normal stem cell morphology and stably expresses pluripotent markers, could provide an ideal platform for exploring the role of DKK2 in embryonic development. In addition, Zeocin selection combined with tiny clone picking might be a highly efficient way to generate gene-knockout hESC lines.
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页数：5
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