Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system

被引：0
|
作者：
Zhang, Canwei [1 ]
Yu, Yankun [1 ]
Li, Fuxi [1 ]
Lan, Xihong [1 ]
Wang, Li [1 ]
机构：
[1] Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510623, Peoples R China
来源：
STEM CELL RESEARCH | 2020年 / 49卷
基金：
中国国家自然科学基金; 中国博士后科学基金;
关键词：
D O I：
10.1016/j.scr.2020.102069
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Myeloid ecotropic viral integration site 1 (MEIS1) plays an essential role in the development of several embryonic organs, such as the central nervous system and eyes. To further investigate the role of MEIS1 in embryonic development, herein, we generated a MEIS1 homozygous knockout human embryonic stem cell (hESC) line using the CRISPR/Cas9 genome-editing technology. We believe that this cell line will be a good resource for exploring the function of the MEIS1 gene in embryonic development in vitro. Furthermore, the gene-knockout method reported in this study is efficient and labor-saving, which may provide an effective strategy for hESC gene deletion.
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页数：5
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