The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system

被引：2
|
作者：
Dong, Tao [1 ,2 ]
Zhang, Siyao [1 ,2 ]
Chang, Yun [1 ,2 ]
Bai, Rui [1 ,2 ]
Jiang, Youxu [1 ,2 ]
Ma, Shuhong [1 ,2 ]
Li, Yanan [1 ,2 ]
Jiang, Hong Feng [1 ,2 ]
Lu, Wen-Jing [1 ,2 ]
机构：
[1] Capital Med Univ, Beijing Collaborat Innovat Ctr Cardiovasc Disorde, Beijing Lab Cardiovasc Precis Med,Minist Educ, Anzhen Hosp,Key Lab Remodeling Related Cardiovasc, Beijing 100029, Peoples R China
[2] Beijing Inst Heart, Lung & Blood Vessel Dis, Beijing 100029, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 51卷
基金：
中国国家自然科学基金; 北京市自然科学基金;
关键词：
D O I：
10.1016/j.scr.2021.102196
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
SNTA1 encodes alpha 1-syntrophin, a scaffold protein, which is a component of the dystrophin-associated protein complex. Additionally, a1-syntrophin interacts with SCN5A and nNOS-PMCA4b complex in cardiomyocytes. SNTA1 is a susceptibility locus for arrhythmia and cardiomyopathy. We generated a homozygous SNTA1 knockout human embryonic stem cell (H9SNTA1KO) using the CRISPR/Cas9 system. H9SNTA1KO maintained pluripotency and a normal karyotype and differentiated into three germ layers in vivo.
引用
收藏
页数：4
相关论文
共 50 条
  • [1] Generation of a FMR1 homozygous knockout human embryonic stem cell line (WAe009-A-16) by CRISPR/Cas9 editing
    Giri, Subhajit
    Purushottam, Meera
    Viswanath, Biju
    Muddashetty, Ravi S.
    [J]. STEM CELL RESEARCH, 2019, 39
  • [2] Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system
    Zhang, Shuai
    Guo, Tianwei
    Song, Yuanxiu
    Jiang, Mengqi
    Jiang, Youxu
    Dong, Tao
    Zhang, Siyao
    Wang, Hong
    Hou, Xiaotong
    [J]. STEM CELL RESEARCH, 2022, 59
  • [3] Generating dHAND homozygous knockout human embryonic stem cell line (WAe009-A-59) by episomal vector-based CRISPR/Cas9 system
    Wang, Wei
    Bai, Rui
    Song, Xiantao
    [J]. STEM CELL RESEARCH, 2021, 55
  • [4] Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
    Xiao, Xiuqing
    Chen, Yashuang
    Peng, Lisheng
    Zhang, Tian
    [J]. STEM CELL RESEARCH, 2021, 53
  • [5] Generation of a DKK1 homozygous knockout human embryonic stem cell line WAe001-A-21 using CRISPR/Cas9
    Yang, Zhen
    Yuan, Fang
    Wang, Ning
    Liu, Yanli
    Zhang, Jiaye
    Wei, Ruzhi
    Wu, FeiMa
    Wu, Yuhang
    You, Kai
    Chen, Yan
    Yang, Fan
    Li, Yin-Xiong
    [J]. STEM CELL RESEARCH, 2021, 51
  • [6] Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 genome editing
    Zhao, Tiantian
    Bai, Rui
    Wu, Fujian
    Lu, Wen-Jing
    Zhang, Jun
    [J]. STEM CELL RESEARCH, 2021, 51
  • [7] Generation of SHMT2 knockout human embryonic stem cell line (WAe009-A-67) using CRISPR/Cas9 technique
    Li, Jisheng
    Zhang, Bowen
    Gan, Min
    Li, Yunxing
    He, Lijuan
    Yue, Wen
    Qiao, Haixuan
    Pei, Xuetao
    Li, Yanhua
    [J]. STEM CELL RESEARCH, 2021, 57
  • [8] Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
    Zhang, Canwei
    Yu, Yankun
    Li, Fuxi
    Lan, Xihong
    Wang, Li
    [J]. STEM CELL RESEARCH, 2020, 49
  • [9] Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system
    You, Jie
    Xi, Hairui
    Ma, Shuangping
    Yang, Xian-Jie
    Chen, Ling
    [J]. STEM CELL RESEARCH, 2021, 56
  • [10] Generation of a SMO homozygous knockout human embryonic stem cell line WAe001-A-16 by CRISPR/Cas9 editing
    Wu, Feima
    Gao, Ge
    Pan, Tingcai
    Yang, Zhen
    Xu, Guosheng
    Abbas, Nasir
    Liu, Yanli
    Chen, Yan
    Tan, Shenglin
    You, Kai
    Ke, Xinrong
    Zhuang, Yuanqi
    Lin, Xianhua
    Yang, Fan
    Li, Yin-xiong
    [J]. STEM CELL RESEARCH, 2018, 27 : 5 - 9
12345