The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system

被引：2
|
作者：
Dong, Tao [1 ,2 ]
Zhang, Siyao [1 ,2 ]
Chang, Yun [1 ,2 ]
Bai, Rui [1 ,2 ]
Jiang, Youxu [1 ,2 ]
Ma, Shuhong [1 ,2 ]
Li, Yanan [1 ,2 ]
Jiang, Hong Feng [1 ,2 ]
Lu, Wen-Jing [1 ,2 ]
机构：
[1] Capital Med Univ, Beijing Collaborat Innovat Ctr Cardiovasc Disorde, Beijing Lab Cardiovasc Precis Med,Minist Educ, Anzhen Hosp,Key Lab Remodeling Related Cardiovasc, Beijing 100029, Peoples R China
[2] Beijing Inst Heart, Lung & Blood Vessel Dis, Beijing 100029, Peoples R China
来源：
STEM CELL RESEARCH | 2021年 / 51卷
基金：
中国国家自然科学基金; 北京市自然科学基金;
关键词：
D O I：
10.1016/j.scr.2021.102196
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
SNTA1 encodes alpha 1-syntrophin, a scaffold protein, which is a component of the dystrophin-associated protein complex. Additionally, a1-syntrophin interacts with SCN5A and nNOS-PMCA4b complex in cardiomyocytes. SNTA1 is a susceptibility locus for arrhythmia and cardiomyopathy. We generated a homozygous SNTA1 knockout human embryonic stem cell (H9SNTA1KO) using the CRISPR/Cas9 system. H9SNTA1KO maintained pluripotency and a normal karyotype and differentiated into three germ layers in vivo.
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页数：4
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