Generation of a TIMP3 knockout stem cell line via CRISPR/Cas9 system

被引：0
|
作者：
Yi, Tong [1 ]
Liu, Jingjing [1 ]
Zhang, Siyao [1 ]
He, Yihua [1 ,2 ]
Han, Jiancheng [1 ,2 ]
机构：
[1] Capital Med Univ, Beijing Anzhen Hosp, Dept Ultrasound, Beijing Key Lab Maternal Fetus Med Fetal Heart Dis, Beijing, Peoples R China
[2] Beijing Anzhen Hosp, Dept Ultrasound, Beijing Key Lab Maternal Fetus Med Fetal Heart Dis, 2 Anzhen Rd, Beijing 100029, Peoples R China
来源：
STEM CELL RESEARCH | 2023年 / 67卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2023.103034
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
The tissue inhibitors of metalloproteinases 3 (TIMP3) play an essential role in the tumorigenesis of human pancreatic endocrine tumors and Sorsby fundus dystrophy. To further investigate the significance of TIMP3 in disease, we used CRISPR/Cas9 to create a TIMP3 knock out human embryonic stem cell line (WAe009-A-89) that can differentiate into any desired cell type. Our results show that the WAe009-A-89 cell line retains the typical colony form and normal karyotype of stem cells. The cells strongly expressed pluripotency markers and could differentiate into tissues of all three germ layers in vivo. This cell line allowed exploring the role of the TIMP3 gene in related diseases.
引用
收藏
页数：4
相关论文
共 50 条
  • [41] Generation of androgen receptor knockout chicken using CRISPR/Cas9 system
    Lengyel, K.
    Eroglu, I.
    Hellmich, R.
    Sid, H.
    Gahr, M.
    Schusser, B.
    TRANSGENIC RESEARCH, 2020, 29 (04) : 478 - 479
  • [42] One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
    Hongbing HAN
    Yonghe MA
    Tao WANG
    Ling LIAN
    Xiuzhi TIAN
    Rui HU
    Shoulong DENG
    Kongpan LI
    Feng WANG
    Ning LI
    Guoshi LIU
    Yaofeng ZHAO
    Zhengxing LIAN
    Frontiers of Agricultural Science and Engineering, 2014, 1 (01) : 2 - 5
  • [43] One-step generation of myostatin gene knockout sheep via the CRISPR/Cas9 system
    Han, Hongbing
    Ma, Yonghe
    Wang, Tao
    Lian, Ling
    Tian, Xiuzhi
    Hu, Rui
    Deng, Shoulong
    Li, Kongpan
    Wang, Feng
    Li, Ning
    Liu, Guoshi
    Zhao, Yaofeng
    Lian, Zhengxing
    FRONTIERS OF AGRICULTURAL SCIENCE AND ENGINEERING, 2014, 1 (01) : 2 - 5
  • [44] RAPID COMMUNICATION : Generation of FGF5 knockout sheep via the CRISPR/Cas9 system
    Hu, R.
    Fan, Z. Y.
    Wang, B. Y.
    Deng, S. L.
    Zhang, X. S.
    Zhang, J. L.
    Han, H. B.
    Lian, Z. X.
    JOURNAL OF ANIMAL SCIENCE, 2017, 95 (05) : 2019 - 2024
  • [45] Generation of an S100B homozygous knockout pluripotent stem cell line (WAe009-A-94) by the CRISPR/Cas9 system
    Zhang, Pengxia
    Wang, Hongyue
    Li, Shengzhen
    Guo, Tianwei
    Zhang, Rui
    Zhou, Ping
    Ding, Shaoli
    STEM CELL RESEARCH, 2022, 64
  • [46] Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system
    You, Jie
    Xi, Hairui
    Ma, Shuangping
    Yang, Xian-Jie
    Chen, Ling
    STEM CELL RESEARCH, 2021, 56
  • [47] Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system
    You, Jie
    Cheng, Yun
    Yang, Xian-Jie
    Chen, Ling
    STEM CELL RESEARCH, 2021, 53
  • [48] Generation of a homozygous ALX1 knockout human embryonic stem cell line (WAe001-A-060) by a CRISPR/Cas9 system
    Xiao, Xiuqing
    Chen, Yashuang
    Peng, Lisheng
    Zhang, Tian
    STEM CELL RESEARCH, 2021, 53
  • [49] Generation of a homozygous FIS1 knockout human embryonic stem cell line GIBHe015-B by CRISPR/Cas9 system
    Wang, Mingquan
    Tang, Jun
    Zhou, Tiancheng
    Mai, Yuchan
    Zhu, Yanling
    STEM CELL RESEARCH, 2022, 65
  • [50] Generating an MEIS1 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
    Zhang, Canwei
    Yu, Yankun
    Li, Fuxi
    Lan, Xihong
    Wang, Li
    STEM CELL RESEARCH, 2020, 49
12345