PURIFICATION AND PROPERTIES OF 2 FORMS OF GLUCOAMYLASE FROM SACCHAROMYCOPSIS-FIBULIGERA

被引：11

作者：

FUTATSUGI, M ^{[1
]}

OGAWA, T ^{[1
]}

FUKUDA, H ^{[1
]}

机构：

[1] KUMAMOTO INST TECHNOL,DEPT APPL MICROBIAL TECHNOL,KUMAMOTO 860,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 76卷 / 06期

关键词：

D O I：

10.1016/0922-338X(93)90252-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two forms of glucoamylase were formed in the filtrate of a 6-d culture of Saccharomycopsis fibuligera that had been cultivated under aerobic conditions with an oxygen-transfer rate of 35x10(-7) g mol/ml/min. The glucoamylases, designated as I and II, resembled each other in termes of pH stability, optimum temperature and the effects of various chemicals on enzymatic activity, but the molecular weights (55,000 and 56,000, respectively), isoelectric points (6.5 and 6.1, respectively), thermal stability, and K, values for maltose differed between the two enzymes.

引用

页码：521 / 523

页数：3

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