Evaluation of the glucoamylase Glm from Saccharomycopsis fibuligera IFO 0111 in hydrolysing the corn starch

The starch hydrolysis is an important step in the processes of starch technologies and treatment of starch substrates. High attention has been paid to the industrial hydrolysis of starch substrates by enzymatic preparations. In this work the glucoamylase Glm from Saccharomycopsis fibuligera IFO 0111 was used in an attempt to obtain starch hydrolysates. This enzyme very probably possesses the raw starch-binding site within the structure of catalytic domain allowing it to split the native (i.e. raw) starch. The main goal of the present work was to test the S. fibuligera 1170 0111 glucoamylase in hydrolysing the raw starches. Two substrates were used: starch milk and corn starch (initial concentration 23% w/w). The effects of enzyme dosage and thermal processing of substrates on kinetics and efficiency of hydrolysis were observed. The results suggested that the glucoamylase was sufficiently effective in hydrolysing tested raw starches and thermal modification of substrates was not necessary.