PURIFICATION AND PROPERTIES OF 2 FORMS OF GLUCOAMYLASE FROM SACCHAROMYCOPSIS-FIBULIGERA

被引：11
|
作者：
FUTATSUGI, M [1 ]
OGAWA, T [1 ]
FUKUDA, H [1 ]
机构：
[1] KUMAMOTO INST TECHNOL,DEPT APPL MICROBIAL TECHNOL,KUMAMOTO 860,JAPAN
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 76卷 / 06期
关键词：
D O I：
10.1016/0922-338X(93)90252-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two forms of glucoamylase were formed in the filtrate of a 6-d culture of Saccharomycopsis fibuligera that had been cultivated under aerobic conditions with an oxygen-transfer rate of 35x10(-7) g mol/ml/min. The glucoamylases, designated as I and II, resembled each other in termes of pH stability, optimum temperature and the effects of various chemicals on enzymatic activity, but the molecular weights (55,000 and 56,000, respectively), isoelectric points (6.5 and 6.1, respectively), thermal stability, and K, values for maltose differed between the two enzymes.
引用
收藏
页码：521 / 523
页数：3
相关论文
共 50 条
  • [1] SCALE-UP OF GLUCOAMYLASE PRODUCTION BY SACCHAROMYCOPSIS-FIBULIGERA
    FUTATSUGI, M
    OGAWA, T
    FUKUDA, H
    [J]. JOURNAL OF FERMENTATION AND BIOENGINEERING, 1993, 76 (05): : 419 - 422
  • [2] PURIFICATION AND CHARACTERIZATION OF THE AMYLOLYTIC ENZYMES OF SACCHAROMYCOPSIS-FIBULIGERA
    GASPERIK, J
    KOVAC, L
    MINARIKOVA, O
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1991, 23 (01): : 21 - 25
  • [3] CLONING AND EXPRESSION OF THE SACCHAROMYCOPSIS-FIBULIGERA GLUCOAMYLASE GENE IN SACCHAROMYCES-CEREVISIAE
    YAMASHITA, I
    ITOH, T
    FUKUI, S
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1985, 23 (02) : 130 - 133
  • [4] THE NUCLEOTIDE-SEQUENCE OF THE GLUCOAMYLASE GENE GLA1 FROM SACCHAROMYCOPSIS-FIBULIGERA KZ
    HOSTINOVA, E
    BALANOVA, J
    GASPERIK, J
    [J]. FEMS MICROBIOLOGY LETTERS, 1991, 83 (01) : 103 - 108
  • [5] GLUCOAMYLASES ENCODED BY VARIANT SACCHAROMYCOPSIS-FIBULIGERA GENES - STRUCTURE AND PROPERTIES
    GASPERIK, J
    HOSTINOVA, E
    [J]. CURRENT MICROBIOLOGY, 1993, 27 (01) : 11 - 14
  • [6] PRODUCTION, PURIFICATION AND CHARACTERIZATION OF AN ALPHA-AMYLASE PRODUCED BY SACCHAROMYCOPSIS-FIBULIGERA
    GOGOI, BK
    BEZBARUAH, RL
    PILLAI, KR
    BARUAH, JN
    [J]. JOURNAL OF APPLIED BACTERIOLOGY, 1987, 63 (05): : 373 - 379
  • [7] NUCLEOTIDE-SEQUENCE OF THE GLUCOAMYLASE GENE GLU1 IN THE YEAST SACCHAROMYCOPSIS-FIBULIGERA
    ITOH, T
    OHTSUKI, I
    YAMASHITA, I
    FUKUI, S
    [J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (09) : 4171 - 4176
  • [8] AMYLOLYTIC ENZYMES PRODUCED BY SACCHAROMYCOPSIS-FIBULIGERA
    GASPERIK, J
    HOSTINOVA, E
    MINARIKOVA, O
    SOLDANOVA, I
    ZELINKA, J
    [J]. BIOLOGIA, 1988, 43 (08) : 673 - 679
  • [9] ISOLATION OF DNA FROM THE DIMORPHIC YEAST SACCHAROMYCOPSIS-FIBULIGERA
    HOSTINOVA, E
    BALANOVA, J
    ZELINKA, J
    [J]. BIOLOGIA, 1989, 44 (04) : 307 - 312
  • [10] Structure of glucoamylase from Saccharomycopsis fibuligera at 1.7 Å resolution
    Sevcik, J
    Solovicova, A
    Hostinova, E
    Gasperik, J
    Wilson, K
    Dauter, Z
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 : 854 - 866
12345