PURIFICATION AND PROPERTIES OF 2 FORMS OF GLUCOAMYLASE FROM SACCHAROMYCOPSIS-FIBULIGERA

被引：11
|
作者：
FUTATSUGI, M [1 ]
OGAWA, T [1 ]
FUKUDA, H [1 ]
机构：
[1] KUMAMOTO INST TECHNOL,DEPT APPL MICROBIAL TECHNOL,KUMAMOTO 860,JAPAN
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 76卷 / 06期
关键词：
D O I：
10.1016/0922-338X(93)90252-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two forms of glucoamylase were formed in the filtrate of a 6-d culture of Saccharomycopsis fibuligera that had been cultivated under aerobic conditions with an oxygen-transfer rate of 35x10(-7) g mol/ml/min. The glucoamylases, designated as I and II, resembled each other in termes of pH stability, optimum temperature and the effects of various chemicals on enzymatic activity, but the molecular weights (55,000 and 56,000, respectively), isoelectric points (6.5 and 6.1, respectively), thermal stability, and K, values for maltose differed between the two enzymes.
引用
收藏
页码：521 / 523
页数：3
相关论文
共 50 条
  • [21] EFFECT OF METHIONINE ON THE GROWTH AND MORPHOGENESIS OF SACCHAROMYCOPSIS-FIBULIGERA IN DEFINED MEDIUM
    NWOGUH, CE
    BERRY, DR
    [J]. BIOTECHNOLOGY LETTERS, 1990, 12 (04) : 271 - 276
  • [22] 2-STAGE CONTINUOUS FERMENTATION OF SACCHAROMYCOPSIS-FIBULIGERA AND CANDIDA-UTILIS
    ADMASSU, W
    KORUS, RA
    HEIMSCH, RC
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (11) : 2641 - 2651
  • [23] GROWTH OF SACCHAROMYCOPSIS-FIBULIGERA IN A CONTINUOUS-STIRRED-TANK FERMENTER
    ADMASSU, W
    KORUS, RA
    HEIMSCH, RC
    LEMMEL, SA
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 1981, 23 (10) : 2361 - 2371
  • [24] Biochemical characterization of a glucoamylase from Saccharomycopsis fibuligera R64
    Natalia, Dessy
    Vidilaseris, Keni
    Satrimafitrah, Pasjan
    Purkan, Wangsa T. Ismaya
    Permentier, Hjalmar
    Fibriansah, Guntur
    Puspasari, Fernita
    Nurachman, Zeily
    Dijkstra, Bauke W.
    Soemitro, Soetijoso
    [J]. BIOLOGIA, 2011, 66 (01) : 27 - 32
  • [25] INTERGENERIC HYBRIDS BETWEEN SACCHAROMYCOPSIS-FIBULIGERA AND YARROWIA-LIPOLYTICA
    NGA, BH
    ABUBAKAR, FD
    LOH, GH
    CHIU, LL
    HARASHIMA, S
    OSHIMA, Y
    HESLOT, H
    [J]. JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 : 223 - 227
  • [26] Biochemical characterization of a glucoamylase from Saccharomycopsis fibuligera R64
    Dessy Natalia
    Keni Vidilaseris
    Pasjan Satrimafitrah
    Wangsa T. Ismaya
    Hjalmar Purkan
    Guntur Permentier
    Fernita Fibriansah
    Zeily Puspasari
    Bauke W. Nurachman
    Soetijoso Dijkstra
    [J]. Biologia, 2011, 66 : 27 - 32
  • [27] ATPASE ACTIVITY IN CELLS OF THE YEAST SACCHAROMYCOPSIS-FIBULIGERA DURING MYCELIAL GROWTH
    OTA, A
    MORISHITA, H
    [J]. MICROBIOS, 1988, 53 (216-17) : 147 - 159
  • [28] FERMENTATION OF STARCH TO ETHANOL BY A COCULTURE OF SACCHAROMYCOPSIS-FIBULIGERA AND SACCHAROMYCES-CEREVISIAE
    PIRSELOVA, K
    SMOGROVICOVA, D
    BALAZ, S
    [J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1993, 9 (03): : 338 - 341
  • [29] ALPHA-GLUCOSIDASE ACCOMPANYING EXTRACELLULAR AMYLOLYTIC ENZYMES OF SACCHAROMYCOPSIS-FIBULIGERA
    GASPERIK, J
    HOSTINOVA, E
    [J]. BIOLOGIA, 1990, 45 (12) : 1013 - 1019
  • [30] CHARACTERIZATION OF ATPASE ACTIVITY IN THE MYCELIAL FORM CELLS OF YEAST SACCHAROMYCOPSIS-FIBULIGERA
    OTA, A
    MORISHITA, H
    [J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1988, 20 (02): : 147 - &
12345