MODEL FOR THE MIXED ION-EXCLUSION ADSORPTION RETENTION MECHANISM IN ION-EXCLUSION CHROMATOGRAPHY

The model elaborated in a previous paper for the retention mechanism in ion-exclusion chromatography was generalized to include adsorption of the solute. The computer modelling of the column performance by the Craig method was used in the case of an unbuffered mobile phase. The retention process in the case of a sufficiently buffered mobile phase turned out to be governed by a linear partition isotherm and can be described globally by simple equations. The adsorption constants of several compounds were calculated from the data available in the literature.