The retention mechanism of analysed compounds in ion-exclusion chromatography has been described. It is based on the analytical equations and on the results obtained from the computer simulation of the column performance (using global equations or the Craig method) as well as on the experimental data. The influence of some physicochemical parameters that describe the sample, the chromatographic column and the mobile phase on the retention is discussed. Practical applications are briefly presented.
机构:
Univ Ljubljana, Fac Chem & Chem Technol, Ljubljana 1000, SloveniaUniv Ljubljana, Fac Chem & Chem Technol, Ljubljana 1000, Slovenia
Novic, Milko
Haddad, Paul R.
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Univ Tasmania, Australian Ctr Res Separat Sci ACROSS, Sch Chem, Hobart, Tas 7001, AustraliaUniv Ljubljana, Fac Chem & Chem Technol, Ljubljana 1000, Slovenia