LARGE-SCALE GENOMIC SEQUENCING - OPTIMIZATION OF GENOMIC CHEMICAL SEQUENCING REACTIONS

被引：0
|
作者：
DOLAN, M
ALLY, A
PURZYCKI, MS
GILBERT, W
GILLEVET, PM
机构：
[1] LIFE TECHNOL,ROCKVILLE,MD
[2] HARVARD UNIV,CAMBRIDGE,MA 02138
[3] NIH,CAMBRIDGE,MD
来源：
BIOTECHNIQUES | 1995年 / 19卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a streamlined reproducible method for performing genomic chemical sequencing reactions on the genomic DNA of Mycoplasma capricolum, which has a genome size of about 750000 base pairs and whose composition is 75% AT The general modifications that ensure reproducibility and allow the processing of multiple samples can be widely adopted to other large-scale sequencing projects, while the specific modifications to the chemical reactions are applicable to the sequencing of other DNAs with a high AT content.
引用
收藏
页码：264 / &
相关论文
共 50 条
  • [21] Genomic Integrative Pathology: A Large Scale Tumour Next Generation Sequencing Initiative
    Tessier-Cloutier, Basile
    Grewal, Jasleen
    Jones, Martin
    Pleasance, Erin
    Zhong, Ellia Z.
    Mungall, Karen
    Lee, Tae Hoon
    Cai, Ellen
    Sheffield, Brandon
    Lee, Cheng-Han
    Hoang, Lien
    Skinnider, Brian
    Smith, Tyler
    Schaeffer, David
    Lee, Anna F.
    Ng, Tony
    Ionescu, Diana
    Nielsen, Torsten
    Dunham, Chris
    Jones, Steven
    Laskin, Janessa
    Marra, Marco
    Yip, Stephen
    MODERN PATHOLOGY, 2018, 31 : 708 - 708
  • [22] Genomic Integrative Pathology: A Large Scale Tumour Next Generation Sequencing Initiative
    Tessier-Cloutier, Basile
    Grewal, Jasleen
    Jones, Martin
    Pleasance, Erin
    Zhong, Ellia Z.
    Mungall, Karen
    Lee, Tae Hoon
    Cai, Ellen
    Sheffield, Brandon
    Lee, Cheng-Han
    Hoang, Lien
    Skinnider, Brian
    Smith, Tyler
    Schaeffer, David
    Lee, Anna F.
    Ng, Tony
    Ionescu, Diana
    Nielsen, Torsten
    Dunham, Chris
    Jones, Steven
    Laskin, Janessa
    Marra, Marco
    Yip, Stephen
    LABORATORY INVESTIGATION, 2018, 98 : 708 - 708
  • [23] Genomic sequencing in pandemics
    不详
    LANCET, 2021, 397 (10273): : 445 - 445
  • [24] THE COMEBACK OF GENOMIC SEQUENCING
    JORDAN, B
    M S-MEDECINE SCIENCES, 1992, 8 (08): : 854 - 857
  • [25] Genomic sequencing in cancer
    Tuna, Musaffe
    Amos, Christopher I.
    CANCER LETTERS, 2013, 340 (02) : 161 - 170
  • [26] Sequencing of genomic DNA
    Mahlknecht, U
    Hoelzer, D
    Bucala, R
    BIOTECHNIQUES, 1999, 27 (03) : 406 - 408
  • [27] Visualizing large-scale genomic sequences
    Glusman, G
    Lancet, D
    IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 2001, 20 (04): : 49 - 54
  • [28] On the analysis of large-scale genomic structures
    Nestor Norio Oiwa
    Carla Goldman
    Cell Biochemistry and Biophysics, 2005, 42 : 145 - 165
  • [29] On the analysis of large-scale genomic structures
    Oiwa, NN
    Goldman, C
    CELL BIOCHEMISTRY AND BIOPHYSICS, 2005, 42 (02) : 145 - 165
  • [30] Clinical Utility of Large Scale Genomic Sequencing of Solid Tumors at a Large Academic Medical Center
    Brown, N. A.
    Betz, B. L.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2017, 19 (06): : 1021 - 1021
12345