LARGE-SCALE GENOMIC SEQUENCING - OPTIMIZATION OF GENOMIC CHEMICAL SEQUENCING REACTIONS

被引：0
|
作者：
DOLAN, M
ALLY, A
PURZYCKI, MS
GILBERT, W
GILLEVET, PM
机构：
[1] LIFE TECHNOL,ROCKVILLE,MD
[2] HARVARD UNIV,CAMBRIDGE,MA 02138
[3] NIH,CAMBRIDGE,MD
来源：
BIOTECHNIQUES | 1995年 / 19卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a streamlined reproducible method for performing genomic chemical sequencing reactions on the genomic DNA of Mycoplasma capricolum, which has a genome size of about 750000 base pairs and whose composition is 75% AT The general modifications that ensure reproducibility and allow the processing of multiple samples can be widely adopted to other large-scale sequencing projects, while the specific modifications to the chemical reactions are applicable to the sequencing of other DNAs with a high AT content.
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页码：264 / &
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