OPTIMIZATION OF PCR-BASED DIAGNOSTICS FOR HUMAN CYTOMEGALOVIRUS

被引：9
|
作者：
VINOGRADSKAYA, GR
GORYSHIN, IY
BERLIN, YA
LANZOV, VA
机构：
[1] RUSSIAN ACAD SCI,SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA
[2] RUSSIAN ACAD SCI,ST PETERSBURG INST NUCL PHYS,CTR MOLEC DIAGNOST,GATCHINA,RUSSIA
[3] RUSSIAN MINIST HLTH,ST PETERSBURG PAEDIAT MED INST,ST PETERSBURG,RUSSIA
来源：
JOURNAL OF VIROLOGICAL METHODS | 1995年 / 53卷 / 01期
关键词：
CMV GENOME; PCR; INTERNAL PRIMER; STRUCTURAL POLYMORPHISM; MOLECULAR EPIDEMIOLOGY;
D O I：
10.1016/0166-0934(95)00002-C
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, deletions, insertions) localized in the analyzed amplicon appear to be suitable for molecular-epidemiological studies. A scheme of spreading of the molecular markers in the population is suggested.
引用
收藏
页码：103 / 112
页数：10
相关论文
共 50 条
  • [41] Development of a PCR-based assay for detection, quantification, and genotyping of human adenoviruses
    Chmielewicz, B
    Nitsche, A
    Schweiger, B
    Ellerbrok, H
    CLINICAL CHEMISTRY, 2005, 51 (08) : 1365 - 1373
  • [42] Comprehensive PCR-based assay for detection and species identification of human herpesviruses
    Johnson, G
    Nelson, S
    Petric, M
    Tellier, R
    JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (09) : 3274 - 3279
  • [43] An optimized method for PCR-based genotyping to detect human APOE polymorphisms
    Najd-Hassan-Bonab, Leila
    Hedayati, Mehdi
    Fazeli, Seyed Abolhassan Shahzadeh
    Daneshpour, Maryam S.
    HELIYON, 2023, 9 (11)
  • [44] A PCR-BASED GENETIC-MAP FOR HUMAN-CHROMOSOME-3
    OCONNELL, P
    LEACH, RJ
    RAINS, D
    TAYLOR, T
    GARCIA, D
    BALLARD, L
    HOLIK, P
    WEISSENBACH, J
    SHERMAN, S
    WILKIE, P
    WEBER, JL
    NAYLOR, SL
    GENOMICS, 1994, 24 (03) : 557 - 567
  • [45] PCR-based Evaluation of Human Papillomavirus Genotypes in Oral Lichen Planus
    Vijayan, Aswathy K.
    Muthukrishnan, Arvind
    Nair, Aparna M.
    Fathima, Shabna
    Nair, Pratheeksha, V
    Roshan, John
    JOURNAL OF PHARMACY AND BIOALLIED SCIENCES, 2022, 14 : 449 - 453
  • [46] Bandstab: A PCR-based alternative to cloning PCR products
    BioTech, 4 (642):
  • [47] Bandstab: A PCR-based alternative to cloning PCR products
    Wilton, SD
    Lim, L
    Dye, D
    Laing, N
    BIOTECHNIQUES, 1997, 22 (04) : 642 - 645
  • [48] Distinction between Enterococcus faecium and Enterococcus lactis by a gluP PCR-Based Assay for Accurate Identification and Diagnostics
    Daza, Mireya Viviana Belloso
    Almeida-Santos, Ana C.
    Novais, Carla
    Read, Antonia
    Alves, Valquiria
    Cocconcelli, Pier Sandro
    Freitas, Ana R.
    Peixe, Luisa
    MICROBIOLOGY SPECTRUM, 2022, 10 (06):
  • [49] PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings
    Yang, S
    Rothman, RE
    LANCET INFECTIOUS DISEASES, 2004, 4 (06): : 337 - 348
  • [50] PCR-based approaches to adduct analysis
    Pfeifer, GP
    Denissenko, MF
    Tang, MS
    TOXICOLOGY LETTERS, 1998, 103 : 447 - 451
12345