OPTIMIZATION OF PCR-BASED DIAGNOSTICS FOR HUMAN CYTOMEGALOVIRUS

被引：9
|
作者：
VINOGRADSKAYA, GR
GORYSHIN, IY
BERLIN, YA
LANZOV, VA
机构：
[1] RUSSIAN ACAD SCI,SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA
[2] RUSSIAN ACAD SCI,ST PETERSBURG INST NUCL PHYS,CTR MOLEC DIAGNOST,GATCHINA,RUSSIA
[3] RUSSIAN MINIST HLTH,ST PETERSBURG PAEDIAT MED INST,ST PETERSBURG,RUSSIA
来源：
JOURNAL OF VIROLOGICAL METHODS | 1995年 / 53卷 / 01期
关键词：
CMV GENOME; PCR; INTERNAL PRIMER; STRUCTURAL POLYMORPHISM; MOLECULAR EPIDEMIOLOGY;
D O I：
10.1016/0166-0934(95)00002-C
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, deletions, insertions) localized in the analyzed amplicon appear to be suitable for molecular-epidemiological studies. A scheme of spreading of the molecular markers in the population is suggested.
引用
收藏
页码：103 / 112
页数：10
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