OPTIMIZATION OF PCR-BASED DIAGNOSTICS FOR HUMAN CYTOMEGALOVIRUS

被引：9

作者：

VINOGRADSKAYA, GR

GORYSHIN, IY

BERLIN, YA

LANZOV, VA

机构：

[1] RUSSIAN ACAD SCI,SHEMYAKIN & OVCHINNIKOV INST BIOORGAN CHEM,MOSCOW 117871,RUSSIA

[2] RUSSIAN ACAD SCI,ST PETERSBURG INST NUCL PHYS,CTR MOLEC DIAGNOST,GATCHINA,RUSSIA

[3] RUSSIAN MINIST HLTH,ST PETERSBURG PAEDIAT MED INST,ST PETERSBURG,RUSSIA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1995年 / 53卷 / 01期

关键词：

CMV GENOME; PCR; INTERNAL PRIMER; STRUCTURAL POLYMORPHISM; MOLECULAR EPIDEMIOLOGY;

D O I：

10.1016/0166-0934(95)00002-C

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, deletions, insertions) localized in the analyzed amplicon appear to be suitable for molecular-epidemiological studies. A scheme of spreading of the molecular markers in the population is suggested.

引用

页码：103 / 112

页数：10

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