A Microchip-Based Method for Rapid Separation of Subclasses of High-Density Lipoprotein

被引:0
|
作者
Huifei Zheng
Hui Cong
Huimin Wang
Qinghui Jin
Jianlong Zhao
机构
[1] Affiliated Hospital of Nantong University,Laboratory Medicine Center
[2] Chinese Academy of Sciences,Shanghai Institute of Microsystem and Information Technology
来源
Chromatographia | 2010年 / 71卷
关键词
Microfluidic chip electrophoresis; High-density lipoprotein subclasses; Coronary heart disease; Assay;
D O I
暂无
中图分类号
学科分类号
摘要
The subclasses of high-density lipoprotein (HDL) labeled with NBD C6-ceramide have been rapidly separated by microfluidic chip electrophoresis and detected by use of laser-induced fluorescence. The subclasses HDL2 and HDL3 were separated in 4 min. Results showed that the HDL2/HDL3 ratio for patients with CHD was much lower than that for healthy subjects. This method could meet the demand for clinical examination of HDL subclasses.
引用
收藏
页码:1119 / 1124
页数:5
相关论文
共 50 条
  • [41] Low-density lipoprotein and high-density lipoprotein particle subclasses predict coronary events and are favorably changed by gemfibrozil therapy in the veterans affairs high-density lipoprotein intervention trial
    Otvos, JD
    Collins, D
    Freedman, DS
    Shalaurova, I
    Schaefer, EJ
    McNamara, JR
    Bloomfield, HE
    Robins, SJ
    CIRCULATION, 2006, 113 (12) : 1556 - 1563
  • [42] High throughput microchip-based separation and quantitation of high-molecular-weight glutenin subunits
    Rhazi, L.
    Bodard, A. -L
    Fathollahi, B.
    Aussenac, T.
    JOURNAL OF CEREAL SCIENCE, 2009, 49 (02) : 272 - 277
  • [43] High-density lipoprotein mimetics: Focus on synthetic high-density lipoprotein
    Shah, Prediman K.
    AMERICAN JOURNAL OF CARDIOLOGY, 2007, 100 (11): : 62N - 67N
  • [44] High-density lipoprotein is a nanoparticle, but not all nanoparticles are high-density lipoprotein
    Cormode, David P.
    Fisher, Edward A.
    Stroes, Erik S. G.
    Mulder, Willem J. M.
    Fayad, Zahi A.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (38) : E3548 - E3548
  • [45] CHARACTERISTICS OF LOW-DENSITY AND HIGH-DENSITY LIPOPROTEIN SUBCLASSES IN PEDIATRIC RENAL TRANSPLANT RECIPIENTS
    Zeljkovic, Aleksandra
    Vekic, Jelena
    Spasojevic-Kalimanovska, Vesna
    Jelic-Ivanovic, Zorana
    Peco-Antic, Amira
    Kostic, Mirjana
    Vasic, Dragan
    Spasic, Slavica
    EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2011, 44 : 191 - 191
  • [46] Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients
    Zeljkovic, Aleksandra
    Vekic, Jelena
    Spasojevic-Kalimanovska, Vesna
    Jelic-Ivanovic, Zorana
    Peco-Antic, Amira
    Kostic, Mirjana
    Vasic, Dragan
    Spasic, Slavica
    TRANSPLANT INTERNATIONAL, 2011, 24 (11) : 1094 - 1102
  • [47] Design and fabrication of an automated microchip-based cell separation device
    Huang, Chengjun
    Yu, Jun
    Zhu, Jiang
    Wang, Lei
    Guo, Min
    ANALYTICAL LETTERS, 2007, 40 (04) : 763 - 778
  • [48] HIGH-DENSITY LIPOPROTEIN
    SCHAEFER, EJ
    ARTERIOSCLEROSIS, 1983, 3 (06): : 629 - 629
  • [49] HIGH-DENSITY LIPOPROTEIN
    不详
    LANCET, 1981, 1 (8218): : 478 - 480
  • [50] 2H2O-Based High-Density Lipoprotein Turnover Method for the Assessment of Dynamic High-Density Lipoprotein Function in Mice
    Kasumov, Takhar
    Willard, Belinda
    Li, Ling
    Li, Min
    Conger, Heather
    Buffa, Jennifer A.
    Previs, Stephen
    McCullough, Arthur
    Hazen, Stanley L.
    Smith, Jonathan D.
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2013, 33 (08) : 1994 - 2003