A Microchip-Based Method for Rapid Separation of Subclasses of High-Density Lipoprotein

被引:0
|
作者
Huifei Zheng
Hui Cong
Huimin Wang
Qinghui Jin
Jianlong Zhao
机构
[1] Affiliated Hospital of Nantong University,Laboratory Medicine Center
[2] Chinese Academy of Sciences,Shanghai Institute of Microsystem and Information Technology
来源
Chromatographia | 2010年 / 71卷
关键词
Microfluidic chip electrophoresis; High-density lipoprotein subclasses; Coronary heart disease; Assay;
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中图分类号
学科分类号
摘要
The subclasses of high-density lipoprotein (HDL) labeled with NBD C6-ceramide have been rapidly separated by microfluidic chip electrophoresis and detected by use of laser-induced fluorescence. The subclasses HDL2 and HDL3 were separated in 4 min. Results showed that the HDL2/HDL3 ratio for patients with CHD was much lower than that for healthy subjects. This method could meet the demand for clinical examination of HDL subclasses.
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页码:1119 / 1124
页数:5
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