A Microchip-Based Method for Rapid Separation of Subclasses of High-Density Lipoprotein

被引:0
|
作者
Huifei Zheng
Hui Cong
Huimin Wang
Qinghui Jin
Jianlong Zhao
机构
[1] Affiliated Hospital of Nantong University,Laboratory Medicine Center
[2] Chinese Academy of Sciences,Shanghai Institute of Microsystem and Information Technology
来源
Chromatographia | 2010年 / 71卷
关键词
Microfluidic chip electrophoresis; High-density lipoprotein subclasses; Coronary heart disease; Assay;
D O I
暂无
中图分类号
学科分类号
摘要
The subclasses of high-density lipoprotein (HDL) labeled with NBD C6-ceramide have been rapidly separated by microfluidic chip electrophoresis and detected by use of laser-induced fluorescence. The subclasses HDL2 and HDL3 were separated in 4 min. Results showed that the HDL2/HDL3 ratio for patients with CHD was much lower than that for healthy subjects. This method could meet the demand for clinical examination of HDL subclasses.
引用
收藏
页码:1119 / 1124
页数:5
相关论文
共 50 条
  • [22] Distribution and correlates of high-density lipoprotein subclasses among children and adolescents
    Freedman, DS
    Bowman, BA
    Srinivasan, SR
    Berenson, GS
    Otvos, JD
    METABOLISM-CLINICAL AND EXPERIMENTAL, 2001, 50 (03): : 370 - 376
  • [23] High-Density Lipoprotein Subclasses, Coronary Artery Disease, and Cardiovascular Mortality
    Silbernagel, Guenther
    Pagel, Philipp
    Pfahlert, Volker
    Genser, Bernd
    Scharnagl, Hubert
    Kleber, Marcus E.
    Delgado, Graciela
    Ohrui, Haruna
    Ritsch, Andreas
    Grammer, Tanja B.
    Koenig, Wolfgang
    Maerz, Winfried
    CLINICAL CHEMISTRY, 2017, 63 (12) : 1886 - 1896
  • [24] Effect of Ezetimibe on Low- and High-Density Lipoprotein Subclasses in Sitosterolemia
    Othman, Rgia
    Myrie, Semone
    Mymin, David
    Merkens, Louise
    Roullet, Jean-Baptiste
    Steiner, Robert
    Jones, Peter
    FASEB JOURNAL, 2015, 29
  • [25] The distribution and correlates of high-density lipoprotein subclasses among children and adolescents
    Freedman, DS
    Bowman, BA
    Srinivasan, SR
    Berenson, GS
    CIRCULATION, 2000, 101 (06) : 735 - 735
  • [26] Effect of ezetimibe on low- and high-density lipoprotein subclasses in sitosterolemia
    Othman, Rgia A.
    Myrie, Semone B.
    Mymin, David
    Roullet, Jean-Baptiste
    Steiner, Robert D.
    Jones, Peter J. H.
    ATHEROSCLEROSIS, 2017, 260 : 27 - 33
  • [27] Abdominal adiposity is associated with high-density lipoprotein subclasses in Japanese schoolchildren
    Okuma, Hiromi
    Okada, Tomoo
    Abe, Yuriko
    Saito, Emiko
    Iwata, Fujihiko
    Hara, Mitsuhiko
    Ayusawa, Mamoru
    Mugishima, Hideo
    Takahashi, Shori
    CLINICA CHIMICA ACTA, 2013, 425 : 80 - 84
  • [28] EFFECTS OF CIMETIDINE AND RANITIDINE ON PLASMA HIGH-DENSITY LIPOPROTEIN SUBCLASSES IN HUMANS
    GRAINGER, SL
    NANJEE, MN
    THOMPSON, RPH
    MILLER, NE
    PHARMACOLOGY, 1988, 36 (06) : 420 - 426
  • [29] Microchip-based human serum atherogenic lipoprotein profile analysis
    Wang, Hua
    Zhang, Wei
    Wan, Jun
    Liu, Weiwei
    Yu, Bo
    Jin, Qinghui
    Guan, Ming
    ANALYTICAL BIOCHEMISTRY, 2014, 467 : 75 - 83
  • [30] SHOULD WE ROUTINELY MEASURE LOW-DENSITY AND HIGH-DENSITY LIPOPROTEIN SUBCLASSES?
    Rizzo, Manfredi
    Berneis, Kaspar
    Zeljkovic, Aleksandra
    Vekic, Jelena
    CLINICAL LABORATORY, 2009, 55 (11-12) : 421 - 429