DNA sequencing and CRISPR-Cas9 gene editing for target validation in mammalian cells

被引：0
|
作者：
Yegor Smurnyy
Mi Cai
Hua Wu
Elizabeth McWhinnie
John A Tallarico
Yi Yang
Yan Feng
机构：
[1] Developmental and Molecular Pathways,
[2] Novartis Institutes for Biomedical Research,undefined
来源：
Nature Chemical Biology | 2014年 / 10卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Drug-resistance mutations provide a classical means to identify biological targets of small molecules. A combination of next-generation DNA sequencing with CRISPR-Cas9 genome editing confirms the targets of 6-thioguanine and triptolide and offers a general approach for target identification in cells.[graphic not available: see fulltext]
引用
收藏
页码：623 / 625
页数：2
相关论文
共 50 条
  • [11] CRISPR-Cas9 gene editing for patients with haemoglobinopathies
    不详
    LANCET HAEMATOLOGY, 2019, 6 (09): : E438 - E438
  • [12] The impact of CRISPR-Cas9 on target identification and validation
    Moore, Jonathan D.
    DRUG DISCOVERY TODAY, 2015, 20 (04) : 450 - 457
  • [13] Applying CRISPR-Cas9 Off-Target Editing on DNA based Steganography
    Zhou, Hong
    Huan, Xiaoli
    INTERNATIONAL JOURNAL OF ADVANCED COMPUTER SCIENCE AND APPLICATIONS, 2019, 10 (08) : 1 - 5
  • [14] Applying CRISPR-Cas9 off-target editing on DNA based steganography
    Zhou H.
    Huan X.
    International Journal of Advanced Computer Science and Applications, 2019, 10 (08): : 1 - 5
  • [15] Application of CRISPR-Cas9 Gene Editing for HIV Host Factor Discovery and Validation
    Cisneros, William J.
    Cornish, Daphne
    Hultquist, Judd F.
    PATHOGENS, 2022, 11 (08):
  • [16] Conditional Genome Editing in the Mammalian Brain Using CRISPR-Cas9
    Sun, Haojie
    Zheng, Jie
    Yi, Ming
    Wan, You
    NEUROSCIENCE BULLETIN, 2021, 37 (03) : 423 - 426
  • [17] Conditional Genome Editing in the Mammalian Brain Using CRISPR-Cas9
    Haojie Sun
    Jie Zheng
    Ming Yi
    You Wan
    NeuroscienceBulletin, 2021, 37 (03) : 423 - 426
  • [18] Conditional Genome Editing in the Mammalian Brain Using CRISPR-Cas9
    Haojie Sun
    Jie Zheng
    Ming Yi
    You Wan
    Neuroscience Bulletin, 2021, 37 : 423 - 426
  • [19] Application of CRISPR-Cas9 gene editing to treat HBV
    Yan, Kun
    Feng, Jiangpeng
    Xiong, Yong
    Chen, Yu
    CHINESE SCIENCE BULLETIN-CHINESE, 2019, 64 (30): : 3142 - 3150
  • [20] CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis
    Rim, John Hoon
    Gopalappa, Ramu
    Gee, Heon Yung
    NEW ENGLAND JOURNAL OF MEDICINE, 2021, 385 (18): : 1722 - 1722
12345