DNA sequencing and CRISPR-Cas9 gene editing for target validation in mammalian cells

被引：0

作者：

Yegor Smurnyy

Mi Cai

Hua Wu

Elizabeth McWhinnie

John A Tallarico

Yi Yang

Yan Feng

机构：

[1] Developmental and Molecular Pathways,

[2] Novartis Institutes for Biomedical Research,undefined

来源：

Nature Chemical Biology | 2014年 / 10卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Drug-resistance mutations provide a classical means to identify biological targets of small molecules. A combination of next-generation DNA sequencing with CRISPR-Cas9 genome editing confirms the targets of 6-thioguanine and triptolide and offers a general approach for target identification in cells.[graphic not available: see fulltext]

引用

页码：623 / 625

页数：2

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