DNA sequencing and CRISPR-Cas9 gene editing for target validation in mammalian cells

被引：0
|
作者：
Yegor Smurnyy
Mi Cai
Hua Wu
Elizabeth McWhinnie
John A Tallarico
Yi Yang
Yan Feng
机构：
[1] Developmental and Molecular Pathways,
[2] Novartis Institutes for Biomedical Research,undefined
来源：
Nature Chemical Biology | 2014年 / 10卷
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摘要：
Drug-resistance mutations provide a classical means to identify biological targets of small molecules. A combination of next-generation DNA sequencing with CRISPR-Cas9 genome editing confirms the targets of 6-thioguanine and triptolide and offers a general approach for target identification in cells.[graphic not available: see fulltext]
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页码：623 / 625
页数：2
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