A resonance light scattering method for determination of DNA using Ru(bpy)2PIP(V)2+

被引：1
|
作者：
Jianping Huang
Fang Chen
Zhike He
机构：
[1] Huazhong University of Science & Technology,Department of Chemistry
[2] Yangtze University,College of Chemistry & Environmental Engineering
[3] Wuhan University,College of Chemistry & Molecular Sciences
来源：
Microchimica Acta | 2007年 / 157卷
关键词：
Key words: DNA; Ru(bpy); PIP(V); ; resonance light scattering.;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel method for DNA detection is proposed that is based on the enhancement of resonance light scattering (RLS) of Ru(bpy)2PIP(V)2+ at pH 2.7. Under optimum conditions, good linear relationships were obtained in the wide concentration range of 16–5120 ng mL−1. The linear equation is IRLS = 13.15 + 171.4 c (µg mL−1), the correlation coefficient (r) is 0.9999. The detection limit of calf thymus DNA is 5.02 ng mL−1. Plasmid DNA extracted from bacillus subtilis was determined by the method with satisfactory results.
引用
收藏
页码：181 / 187
页数：6
相关论文
共 50 条
  • [41] BINDING OF RU(BPY)3(2+) AND RU(PHEN)3(2+) TO POLYNUCLEOTIDES AND DNA - EFFECT OF ADDED SALTS ON THE ABSORPTION AND LUMINESCENCE PROPERTIES
    GORNER, H
    TOSSI, AB
    STRADOWSKI, C
    SCHULTEFROHLINDE, D
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1988, 2 (01) : 67 - 89
  • [42] Photoinitiated DNA binding by cis-[Ru(bpy)2(NH3)2]2+
    Singh, TN
    Turro, C
    INORGANIC CHEMISTRY, 2004, 43 (23) : 7260 - 7262
  • [43] Synthesis, Characterization, and DNA-Binding of Chiral Complexes Δ- and Λ-[Ru(bpy)2(pyip)]2+
    Shi, Shuo
    Yao, Tian-Ming
    Geng, Xiao-Ting
    Jiang, Ling-Feng
    Liu, Jie
    Yang, Qing-Yuan
    Ji, Liang-Nian
    CHIRALITY, 2009, 21 (02) : 276 - 283
  • [44] Turning the [Ru(bpy)2dppz]2+ light-switch on and off with temperature
    Brennaman, MK
    Alstrum-Acevedo, JH
    Fleming, CN
    Jang, P
    Meyer, TJ
    Papanikolas, JM
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (50) : 15094 - 15098
  • [45] Mechanistic Study on the Photochemical "Light Switch" Behavior of [Ru(bpy)2dmdppz]2+
    Wachter, Erin
    Glazer, Edith C.
    JOURNAL OF PHYSICAL CHEMISTRY A, 2014, 118 (45): : 10474 - 10486
  • [46] Electronic Structure and Related Properties of DNA-Molecular Light-Switch Complex [Ru(bpy)2(bopp)]2+
    Li, Jun
    Miao, Ti-Fang
    Liao, Si-Yan
    Zheng, Kang-Cheng
    ASIAN JOURNAL OF CHEMISTRY, 2013, 25 (13) : 7365 - 7370
  • [47] Synthesis, characterization and DNA binding and photocleavage studies of [Ru(bPy)2BDPPZ]2+, [Ru(dmb)2BDPPZ]2+ and [Ru(phen)2BDPPZ]2+ complexes and their antimicrobial activity
    Kumar, K. Ashwini
    Reddy, Kotha Laxma
    Vidhisha, S.
    Satyanarayana, S.
    APPLIED ORGANOMETALLIC CHEMISTRY, 2009, 23 (10) : 409 - 420
  • [48] Electrochemistry of complex formation of carbaryl with ds-DNA using [Ru(bpy)2dppz]2+ as probe
    Yue Xiong
    Tian-Fang Kang
    Li-Ping Lu
    Journal of Solid State Electrochemistry, 2013, 17 : 129 - 136
  • [49] Electrochemistry of complex formation of carbaryl with ds-DNA using [Ru(bpy)2dppz]2+ as probe
    Xiong, Yue
    Kang, Tian-Fang
    Lu, Li-Ping
    JOURNAL OF SOLID STATE ELECTROCHEMISTRY, 2013, 17 (01) : 129 - 136
  • [50] Recent Developments in the Interactions of Classic Intercalated Ruthenium Compounds: [Ru(bpy)2dppz]2+ and [Ru(phen)2dppz]2+ with a DNA Molecule
    Jia, Fuchao
    Wang, Shuo
    Man, Yan
    Kumar, Parveen
    Liu, Bo
    MOLECULES, 2019, 24 (04):
12345