A resonance light scattering method for determination of DNA using Ru(bpy)2PIP(V)2+

被引：1
|
作者：
Jianping Huang
Fang Chen
Zhike He
机构：
[1] Huazhong University of Science & Technology,Department of Chemistry
[2] Yangtze University,College of Chemistry & Environmental Engineering
[3] Wuhan University,College of Chemistry & Molecular Sciences
来源：
Microchimica Acta | 2007年 / 157卷
关键词：
Key words: DNA; Ru(bpy); PIP(V); ; resonance light scattering.;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel method for DNA detection is proposed that is based on the enhancement of resonance light scattering (RLS) of Ru(bpy)2PIP(V)2+ at pH 2.7. Under optimum conditions, good linear relationships were obtained in the wide concentration range of 16–5120 ng mL−1. The linear equation is IRLS = 13.15 + 171.4 c (µg mL−1), the correlation coefficient (r) is 0.9999. The detection limit of calf thymus DNA is 5.02 ng mL−1. Plasmid DNA extracted from bacillus subtilis was determined by the method with satisfactory results.
引用
收藏
页码：181 / 187
页数：6
相关论文
共 50 条
  • [31] A novel method for determination of DNA by use of molecular 'Light Switch' complex of Ru(bipy)2(dppx)2+
    Ling, LS
    He, ZK
    Song, GW
    Yuan, D
    Zeng, YE
    ANALYTICA CHIMICA ACTA, 2000, 403 (1-2) : 209 - 217
  • [32] Selective determination of DNA based on the fluorescence recovery of carbon dots quenched by Ru(bpy)2(dppz)2+
    Zhang, Cengceng
    Hu, Qi
    Wu, Shu
    Chen, Fang
    TALANTA, 2020, 217 (217)
  • [33] SPECTROSCOPIC INVESTIGATION OF DIFFERENTIAL BINDING MODES OF DELTA-RU(BPY)2(PPZ)2+ AND LAMBDA-RU(BPY)2(PPZ)2+ WITH CALF THYMUS DNA
    TYSOE, SA
    MORGAN, RJ
    BAKER, AD
    STREKAS, TC
    JOURNAL OF PHYSICAL CHEMISTRY, 1993, 97 (08): : 1707 - 1711
  • [34] Electronic structures, DNA-binding and related properties of complexes [Ru(bpy)2L]2+ (L = ip, pip, hpip)
    Zheng, KC
    Deng, H
    Liu, XW
    Li, H
    Chao, H
    Ji, LN
    JOURNAL OF MOLECULAR STRUCTURE-THEOCHEM, 2004, 682 (1-3): : 225 - 233
  • [35] Ru(bpy)2(Me2chrysi)2+: A fluorescent reporter of DNA mismatches
    Song, Hang
    Barton, Jacqueline K.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2011, 241
  • [36] Photoluminescence quenching of [Ru(bpy)2(atatp)]2+ bound to a condensed DNA matrix
    Chen, Linlin
    Chao, Hui
    Zhao, Qianwen
    Zheng, Xuling
    Li, Hong
    JOURNAL OF INORGANIC BIOCHEMISTRY, 2016, 154 : 1 - 6
  • [37] ENANTIOMERIC RESOLUTION OF RU(PHEN)3(2+) AND RU(BPY)2PPZ2+ ON A DNA HYDROXYLAPATITE COLUMN
    BAKER, AD
    MORGAN, RJ
    STREKAS, TC
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (04) : 1411 - 1412
  • [38] Long-range resonance energy transfer to [Ru(bpy)3]2+
    Tyson, DS
    Gryczynski, I
    Castellano, FN
    JOURNAL OF PHYSICAL CHEMISTRY A, 2000, 104 (13): : 2919 - 2924
  • [39] Determination of DNA by Use of the Molecular “Light Switch” Complex of Ru(bipy)2(dppz)2+
    Lian-Sheng Ling
    Zhi-Ke He
    Gong-Wu Song
    He-You Han
    Hua-Shan Zhang
    Yun-E Zeng
    Microchimica Acta, 2000, 134 : 57 - 62
  • [40] Determination of DNA by use of the molecular "Light Switch" complex of Ru(bipy)2(dppz)2+
    Ling, LS
    He, ZK
    Song, GW
    Han, HY
    Zhang, HS
    Zeng, YE
    MIKROCHIMICA ACTA, 2000, 134 (1-2) : 57 - 62
12345