A resonance light scattering method for determination of DNA using Ru(bpy)2PIP(V)2+

被引：1
|
作者：
Jianping Huang
Fang Chen
Zhike He
机构：
[1] Huazhong University of Science & Technology,Department of Chemistry
[2] Yangtze University,College of Chemistry & Environmental Engineering
[3] Wuhan University,College of Chemistry & Molecular Sciences
来源：
Microchimica Acta | 2007年 / 157卷
关键词：
Key words: DNA; Ru(bpy); PIP(V); ; resonance light scattering.;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A novel method for DNA detection is proposed that is based on the enhancement of resonance light scattering (RLS) of Ru(bpy)2PIP(V)2+ at pH 2.7. Under optimum conditions, good linear relationships were obtained in the wide concentration range of 16–5120 ng mL−1. The linear equation is IRLS = 13.15 + 171.4 c (µg mL−1), the correlation coefficient (r) is 0.9999. The detection limit of calf thymus DNA is 5.02 ng mL−1. Plasmid DNA extracted from bacillus subtilis was determined by the method with satisfactory results.
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页码：181 / 187
页数：6
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