Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection

被引：0
|
作者：
Rika Shiota
Hirotoshi Morita
Tomoko Matsumoto
Atsushi Morimoto
Jun Hayakawa
Masako Oka
Hiroshi Kamimori
机构：
[1] Shionogi TechnoAdvance Research Co.,Pharmaceutical Research Division
[2] Ltd.,undefined
[3] Biomarker R&D Department,undefined
[4] Shionogi & Co. Ltd.,undefined
[5] Shionogi & Co. Ltd,undefined
来源：
Analytical Sciences | 2017年 / 33卷
关键词：
Hydroxyproline; mass spectrometry; mouse; kidney;
D O I：
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学科分类号：
摘要：
An analytical method was developed and validated for the measurement of hydroxyproline (Hyp) levels in mouse kidney by high-performance liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) using an analytical column specially designed for the LC/MS/MS analysis for intact amino acids. Tissue hydrolyzed with hydrochloric acid could be directly injected into the LC/MS/MS, as well as separated and detected using the deuterium labelled Hyp as an internal standard. The calibration curve showed good linearity from 5 to 500 nmol/mg of tissue; the precision and accuracy, including within- and between-run, were less than 6% and within 100 ± 6%, respectively.
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页码：719 / 722
页数：3
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