Bioanalytical Method for the Determination of Hydroxyproline in Mouse Kidney by High-Performance Liquid Chromatography with Tandem Mass Spectrometric Detection

被引：0

作者：

Rika Shiota

Hirotoshi Morita

Tomoko Matsumoto

Atsushi Morimoto

Jun Hayakawa

Masako Oka

Hiroshi Kamimori

机构：

[1] Shionogi TechnoAdvance Research Co.,Pharmaceutical Research Division

[2] Ltd.,undefined

[3] Biomarker R&D Department,undefined

[4] Shionogi & Co. Ltd.,undefined

[5] Shionogi & Co. Ltd,undefined

来源：

Analytical Sciences | 2017年 / 33卷

关键词：

Hydroxyproline; mass spectrometry; mouse; kidney;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

An analytical method was developed and validated for the measurement of hydroxyproline (Hyp) levels in mouse kidney by high-performance liquid chromatography with tandem mass spectrometric detection (LC/MS/MS) using an analytical column specially designed for the LC/MS/MS analysis for intact amino acids. Tissue hydrolyzed with hydrochloric acid could be directly injected into the LC/MS/MS, as well as separated and detected using the deuterium labelled Hyp as an internal standard. The calibration curve showed good linearity from 5 to 500 nmol/mg of tissue; the precision and accuracy, including within- and between-run, were less than 6% and within 100 ± 6%, respectively.

引用

页码：719 / 722

页数：3

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