High-performance liquid chromatographic-tandem mass spectrometric method for the determination of clemastine in human plasma

被引：10
|
作者：
Horváth, V
Tolokán, A
Egresi, A
Pap, T
Horvai, G
Balogh-Nernes, K
Klebovich, I
机构：
[1] Budapest Univ Technol & Econ, Hungarian Acad Sci, Res Grp Tech Analyt Chem, H-1111 Budapest, Hungary
[2] Dr E Wessling Chem Lab Ltd, H-1047 Budapest, Hungary
[3] Budapest Univ Technol & Econ, Dept Chem Informat Technol, H-1111 Budapest, Hungary
[4] EGIS Pharmaceut Ltd, Dept Chem Informat Technol, H-1106 Budapest, Hungary
[5] EGIS Pharmaceut Ltd, Dept Pharmacokinet, H-1106 Budapest, Hungary
[6] Semmelweis Univ, Dept Pharmaceut, H-1092 Budapest, Hungary
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 816卷 / 1-2期
关键词：
high-performance liquid chromatography-tandem mass spectrometry; clemastine; antihistamine;
D O I：
10.1016/j.jchromb.2004.11.025
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A highly sensitive high-performance liquid chromatographic-tandem mass spectrometric method (HPLC-MS-MS) has been developed to quantitate clemastine in human plasma for the purpose of pharmacokinetic studies. Sample preparation was carried out by liquid-liquid extraction using deuterated clemastine as an internal standard. Chromatographic separation used a C18 reversed phase polymer column giving an extremely fast total run time of 2 min. The method was validated and used for the bioequivalence study of clemastine tablets in healthy male volunteers (n = 28). The lower limit of detection proved to be 0.01 ng/ml for clemastine. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：153 / 159
页数：7
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