Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

被引：0
|
作者：
Fengjue Shu
Shuguang Guo
Yongjun Dang
Meiyan Qi
Guangjin Zhou
Zekun Guo
Ying Zhang
Chaoqun Wu
Shouyuan Zhao
Long Yu
机构：
[1] Fudan University,State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science
来源：
Molecular and Cellular Biochemistry | 2003年 / 254卷
关键词：
Aurora-B; degradation; HC8; mitosis; proteasome;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Human Aurora/Ipl1-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.
引用
收藏
页码：157 / 162
页数：5
相关论文
共 50 条
  • [31] Human cytomegalovirus pp71 stimulates cell cycle progression by inducing the proteasome-dependent degradation of the retinoblastoma family of tumor suppressors
    Kalejta, RF
    Bechtel, JT
    Shenk, T
    MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (06) : 1885 - 1895
  • [32] Inhibition of proteasome-dependent degradation of Wee1 in G2-arrested Hep3B cells by TGFβ1
    Hashimoto, O
    Ueno, T
    Kimura, R
    Ohtsubo, M
    Nakamura, T
    Koga, H
    Torimura, T
    Uchida, S
    Yamashita, K
    Sata, M
    MOLECULAR CARCINOGENESIS, 2003, 36 (04) : 171 - 182
  • [33] Estrogen-Dependent Gene Transcription in Human Breast Cancer Cells Relies upon Proteasome-Dependent Monoubiquitination of Histone H2B
    Prenzel, Tanja
    Begus-Nahrmann, Yvonne
    Kramer, Frank
    Hennion, Magali
    Hsu, Chieh
    Gorsler, Theresa
    Hintermair, Corinna
    Eick, Dirk
    Kremmer, Elisabeth
    Simons, Mikael
    Beissbarth, Tim
    Johnsen, Steven A.
    CANCER RESEARCH, 2011, 71 (17) : 5739 - 5753
  • [34] Herpes simplex virus type 1 immediate-early protein Vmw110 induces the proteasome-dependent degradation of the catalytic subunit of DNA-dependent protein kinase
    Parkinson, J
    Lees-Miller, SP
    Everett, RD
    JOURNAL OF VIROLOGY, 1999, 73 (01) : 650 - 657
  • [35] Lysine 269 is essential for cyclin D1 ubiquitylation by the SCFFbx4/αB-crystallin ligase and subsequent proteasome-dependent degradation
    Barbash, O.
    Egan, E.
    Pontano, L. L.
    Kosak, J.
    Diehl, J. A.
    ONCOGENE, 2009, 28 (49) : 4317 - 4325
  • [36] Lysine 269 is essential for cyclin D1 ubiquitylation by the SCFFbx4/αB-crystallin ligase and subsequent proteasome-dependent degradation
    O Barbash
    E Egan
    L L Pontano
    J Kosak
    J A Diehl
    Oncogene, 2009, 28 : 4317 - 4325
  • [37] Nuclear Export Signal of Androgen Receptor (NESAR) Regulation of Androgen Receptor Level in Human Prostate Cell Lines via Ubiquitination and Proteasome-Dependent Degradation
    Gong, Yanqing
    Wang, Dan
    Dar, Javid A.
    Singh, Prabhpreet
    Graham, Lara
    Liu, Weijun
    Ai, Junkui
    Xin, Zhongcheng
    Guo, Yinglu
    Wang, Zhou
    ENDOCRINOLOGY, 2012, 153 (12) : 5716 - 5725
  • [38] p97/VCP promotes Cullin-RING-ubiquitin-ligase/proteasome-dependent degradation of IκBα and the preceding liberation of RelA from ubiquitinated IκBα
    Schweitzer, Katrin
    Pralow, Alexander
    Naumann, Michael
    JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2016, 20 (01) : 58 - 70
  • [39] Docosahexaenoic acid induces proteasome-dependent degradation of β-catenin, down-regulation of survivin and apoptosis in human colorectal cancer cells not expressing COX-2
    Calviello, Gabriella
    Resci, Federica
    Serini, Simona
    Piccioni, Elisabetta
    Toesca, Amelia
    Boninsegna, Alma
    Monego, Giovanni
    Ranelletti, Franco O.
    Palozza, Paola
    CARCINOGENESIS, 2007, 28 (06) : 1202 - 1209
  • [40] Altersolanol B, a fungal metabolite, induces proteasome-dependent degradation of estrogen receptor a (ERα) and inhibits downstream signaling targets in ER plus breast adenocarcinoma cells
    Siraj, Md Afjalus
    Rahman, Md Sajjadur
    Jacobs, Aaron T.
    Tan, Ghee T.
    CANCER RESEARCH, 2023, 83 (05)
12345