Purification of recombinant Bacillus cereus ResD-ResE proteins expressed in Escherichia coli strains

被引：0
|
作者：
E. V. Shapyrina
A. M. Shadrin
A. S. Solonin
机构：
[1] Russian Academy of Sciences,Skryabin Institute of Biochemistry and Physiology of Microorganisms
[2] Imperial College,undefined
来源：
Applied Biochemistry and Microbiology | 2013年 / 49卷
关键词：
Apply Biochemistry; Bacillus Cereus; Brain Heart Infusion; Codon Adapta Tion Index; Page Electrophoresis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Recombinant E. coli strains expressing the Bacillus cereus ATCC 14579TresD and resE genes fused with the ubiquitin gene were constructed, and purification of the ResD and ResE proteins was performed. The approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous ResD and ResE proteins without denaturation steps up to 150 mg per gram of wet cell weight.
引用
收藏
页码：542 / 548
页数：6
相关论文
共 50 条
  • [41] PURIFICATION OF RECOMBINANT HUMAN INTERFERON-BETA EXPRESSED IN ESCHERICHIA-COLI
    LIN, LS
    YAMAMOTO, R
    DRUMMOND, RJ
    METHODS IN ENZYMOLOGY, 1986, 119 : 183 - 192
  • [42] Purification and characterization of biologically active recombinant human Eppin expressed in Escherichia coli
    ZHU QingyiGU XiaojianYANG JinWANG JunhongTANG Bo and WU Hongfei Department of UrologyFirst Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu China Department of UrologyJiangsu Province Hospital of Traditional Chinese MedicineNanjingJiangsu China Department of BiochemistrySchool of Life ScienceNanjing UniversityNanjingJiangsu China
    中华医学杂志（英文版）, 2008, (07) : 620 - 624
  • [43] RECOMBINANT HUMAN ACETYLCHOLINESTERASE EXPRESSED IN ESCHERICHIA-COLI - REFOLDING, PURIFICATION AND CHARACTERIZATION
    FISCHER, M
    ITTAH, A
    GORECKI, M
    WERBER, MM
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1995, 21 : 295 - 311
  • [44] Purification and in vitro folding of recombinant human thrombopoietin receptor expressed in Escherichia coli
    Ng, CKF
    Huxtable, S
    Xu, PL
    Hsieh, DPH
    PROTEIN EXPRESSION AND PURIFICATION, 2001, 21 (01) : 129 - 133
  • [45] EFFECT OF ALBOMYCIN (GRISEIN) ON SYNTHESIS OF NUCLEIC ACIDS AND PROTEINS IN BACILLUS CEREUS AND ESCHERICHIA COLI
    VORISEK, J
    GRUNBERG.D
    COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS, 1967, 32 (03) : 1064 - &
  • [46] Purification and characterization of recombinant human interleukin-29 expressed in Escherichia coli
    Li, MC
    He, SH
    JOURNAL OF BIOTECHNOLOGY, 2006, 122 (03) : 334 - 340
  • [47] PURIFICATION OF RECOMBINANT SALMON GROWTH-HORMONE EXPRESSED IN ESCHERICHIA-COLI
    SUGIMOTO, S
    YOKOO, Y
    BIOTECHNOLOGY LETTERS, 1991, 13 (06) : 389 - 394
  • [48] Cloning, purification and characterization of recombinant silkworm arginine kinase expressed in Escherichia coli
    Kang, Lequn
    Shi, Haifeng
    Wang, Nan
    Chen, Yukun
    Yao, Qin
    Zhou, Yajing
    Wang, Yong
    Chen, Huiqing
    Guo, Min
    Wang, Fenghua
    Chen, Keping
    AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011, 10 (36): : 7026 - 7033
  • [49] Purification and characterization of biologically active recombinant human Eppin expressed in Escherichia coli
    Zhu Qing-Yi
    Gu Xiao-Jian
    Yang Jin
    Wang Jun-Hong
    Tang Bo
    Wu Hong-Fei
    CHINESE MEDICAL JOURNAL, 2008, 121 (07) : 620 - 624
  • [50] Purification and characterization of recombinant Plasmodium falciparum adenylosuccinate synthetase expressed in Escherichia coli
    Jayalakshmi, R
    Sumathy, K
    Balaram, H
    PROTEIN EXPRESSION AND PURIFICATION, 2002, 25 (01) : 65 - 72
12345