Purification of recombinant Bacillus cereus ResD-ResE proteins expressed in Escherichia coli strains

被引：0
|
作者：
E. V. Shapyrina
A. M. Shadrin
A. S. Solonin
机构：
[1] Russian Academy of Sciences,Skryabin Institute of Biochemistry and Physiology of Microorganisms
[2] Imperial College,undefined
来源：
Applied Biochemistry and Microbiology | 2013年 / 49卷
关键词：
Apply Biochemistry; Bacillus Cereus; Brain Heart Infusion; Codon Adapta Tion Index; Page Electrophoresis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Recombinant E. coli strains expressing the Bacillus cereus ATCC 14579TresD and resE genes fused with the ubiquitin gene were constructed, and purification of the ResD and ResE proteins was performed. The approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous ResD and ResE proteins without denaturation steps up to 150 mg per gram of wet cell weight.
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页码：542 / 548
页数：6
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