Purification of recombinant Bacillus cereus ResD-ResE proteins expressed in Escherichia coli strains

被引：0
|
作者：
E. V. Shapyrina
A. M. Shadrin
A. S. Solonin
机构：
[1] Russian Academy of Sciences,Skryabin Institute of Biochemistry and Physiology of Microorganisms
[2] Imperial College,undefined
来源：
Applied Biochemistry and Microbiology | 2013年 / 49卷
关键词：
Apply Biochemistry; Bacillus Cereus; Brain Heart Infusion; Codon Adapta Tion Index; Page Electrophoresis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Recombinant E. coli strains expressing the Bacillus cereus ATCC 14579TresD and resE genes fused with the ubiquitin gene were constructed, and purification of the ResD and ResE proteins was performed. The approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous ResD and ResE proteins without denaturation steps up to 150 mg per gram of wet cell weight.
引用
收藏
页码：542 / 548
页数：6
相关论文
共 50 条
  • [31] Isolation of a β-Galactosidase-Encoding Gene from Bacillus licheniformis: Purification and Characterization of the Recombinant Enzyme Expressed in Escherichia coli
    Lam-Son Phan Trân
    Lóránd Szabó
    László Fülöp
    László Orosz
    Tibor Sík
    András Holczinger
    Current Microbiology, 1998, 37 : 39 - 43
  • [32] IMMUNOREACTIVITY OF RECOMBINANT CARCINOEMBRYONIC ANTIGEN PROTEINS EXPRESSED IN ESCHERICHIA-COLI
    KUROKI, M
    MURAKAMI, M
    WAKISAKA, M
    IKEDA, S
    OIKAWA, S
    OSHIMA, T
    NAKAZATO, H
    KOSAKI, G
    MATSUOKA, Y
    IMMUNOLOGICAL INVESTIGATIONS, 1992, 21 (03) : 241 - 257
  • [33] Isolation of a β-galactosidase-encoding gene from Bacillus licheniformis:: Purification and characterization of the recombinant enzyme expressed in Escherichia coli
    Trân, LSP
    Szabó, L
    Fülöp, L
    Orosz, L
    Sík, T
    Holczinger, A
    CURRENT MICROBIOLOGY, 1998, 37 (01) : 39 - 43
  • [34] Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis
    Goncalves, Ana Alice Maia
    Ribeiro, Anna Julia
    Resende, Carlos Ananias Aparecido
    Couto, Carolina Alves Petit
    Gandra, Isadora Braga
    dos Santos Barcelos, Isabelle Caroline
    da Silva, Jonatas Oliveira
    Machado, Juliana Martins
    Silva, Kamila Alves
    Silva, Liria Souza
    dos Santos, Michelli
    Lopes, Lucas da Silva
    de Faria, Mariana Teixeira
    Pereira, Sabrina Paula
    Xavier, Sandra Rodrigues
    Aragao, Matheus Motta
    Candida-Puma, Mayron Antonio
    de Oliveira, Izadora Cristina Moreira
    Souza, Amanda Araujo
    Nogueira, Lais Moreira
    da Paz, Mariana Campos
    Coelho, Eduardo Antonio Ferraz
    Giunchetti, Rodolfo Cordeiro
    de Freitas, Sonia Maria
    Chavez-Fumagalli, Miguel Angel
    Nagem, Ronaldo Alves Pinto
    Galdino, Alexsandro Sobreira
    MICROBIAL CELL FACTORIES, 2024, 23 (01)
  • [35] SOLUBILIZATION, REFOLDING AND PURIFICATION OF EUKARYOTIC PROTEINS EXPRESSED IN ESCHERICHIA-COLI
    LOWE, PA
    RHIND, SK
    SUGRUE, R
    MARSTON, FAO
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1987, : 173 - 173
  • [36] On-chip Escherichia coli culture, purification, and detection of expressed proteins
    Kim, Moonil
    Lee, So-Young
    Choi, Hyunju
    Shin, Yong-Beom
    Jung, Sun Ok
    Kim, Min-Gon
    Chung, Bong Hyun
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2006, 35 (08): : 655 - 662
  • [37] On-chip Escherichia coli culture, purification, and detection of expressed proteins
    Moonil Kim
    So-Young Lee
    Hyunju Choi
    Yong-Beom Shin
    Sun Ok Jung
    Min-Gon Kim
    Bong Hyun Chung
    European Biophysics Journal, 2006, 35 : 655 - 662
  • [38] Purification and characterization of a Bacillus circulans No. 4.1 chitinase expressed in Escherichia coli
    Siwayaprahm, Patcharaporn
    Audtho, Mongkon
    Ohmiya, Kunio
    Wiwat, Chanpen
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2006, 22 (04): : 331 - 335
  • [39] Purification and characterization of a Bacillus circulans No. 4.1 chitinase expressed in Escherichia coli
    Patcharaporn Siwayaprahm
    Mongkon Audtho
    Kunio Ohmiya
    Chanpen Wiwat
    World Journal of Microbiology and Biotechnology, 2006, 22 : 331 - 335
  • [40] A NEW APPROACH FOR PURIFICATION OF RECOMBINANT HUMAN INTERFERON GAMMA EXPRESSED IN ESCHERICHIA COLI
    Petrov, S.
    Ivanova, E.
    Chakarova, D.
    Posheva, V.
    Redzheb, M.
    Nacheva, G.
    Ivanov, I.
    BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2009, 23 (01) : 1101 - 1102
12345