Isolation and characterization of twenty-one polymorphic microsatellite loci for Polycarpa aurata using third generation sequencing

被引：0
|
作者：
Benjamin J. Wainwright
Irma S. Arlyza
Stephen A. Karl
机构：
[1] University of Hawai‘i,Hawai‘i Institute of Marine Biology
[2] Mānoa,Research Center for Oceanography
[3] Indonesian Institute of Sciences,undefined
来源：
Conservation Genetics Resources | 2013年 / 5卷
关键词：
Connectivity; Gene flow; Indonesia; Conservation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Twenty-one microsatellite loci were developed for the tropical ascidian, Polycarpa aurata. Microsatellite enriched genomic libraries were constructed and sequenced using Pacific Biosciences single molecule real time sequencing technology. The 21 loci were characterized in 20 individuals from Alor, Indonesia. Observed levels of heterozygosity ranged from 0.30 to 1.00 with a mean of 8.2 alleles per locus. Loci PA21 and PA45 showed evidence of significant linkage disequilibrium and three loci significantly deviated from Hardy–Weinberg expectations.
引用
收藏
页码：671 / 673
页数：2
相关论文
共 50 条
  • [21] Isolation and characterization of 11 polymorphic microsatellite loci from Fatsia polycarpa (Araliaceae), an element of evergreen forests in Taiwan
    Huang, Chi-Chun
    Hung, Kuo-Hsiang
    Hsu, Tsai-Wen
    Wang, Kuo-Hsiung
    Lin, Chi-Yung
    Chiang, Tzen-Yuh
    CONSERVATION GENETICS, 2008, 9 (05) : 1333 - 1335
  • [22] Isolation and characterization of polymorphic microsatellite loci in Indian major carp, Cada catla using next-generation sequencing platform
    Sahu, B. P.
    Sahoo, L.
    Joshi, C. G.
    Mohanty, P.
    Sundaray, J. K.
    Jayasankar, P.
    Das, P.
    BIOCHEMICAL SYSTEMATICS AND ECOLOGY, 2014, 57 : 357 - 362
  • [23] Isolation and characterization of twenty-four polymorphic microsatellite loci for the topical seagrass, Thalassia hemprichii
    Wainwright, Benjamin J.
    Arlyza, Irma S.
    Karl, Stephen A.
    CONSERVATION GENETICS RESOURCES, 2013, 5 (04) : 939 - 941
  • [24] Isolation and characterization of twenty-four polymorphic microsatellite loci for the topical seagrass, Thalassia hemprichii
    Benjamin J. Wainwright
    Irma S. Arlyza
    Stephen A. Karl
    Conservation Genetics Resources, 2013, 5 : 939 - 941
  • [25] Isolation and characterization of 21 polymorphic microsatellite loci for the rockpool shrimp Palaemon elegans using Illumina MiSeq sequencing
    Gonzalez-Castellano, Ines
    Perina, Alejandra
    Gonzalez-Tizon, Ana M.
    Torrecilla, Zeltia
    Martinez-Lage, Andres
    SCIENTIFIC REPORTS, 2018, 8
  • [26] Isolation and characterization of 21 polymorphic microsatellite loci for the rockpool shrimp Palaemon elegans using Illumina MiSeq sequencing
    Inés González-Castellano
    Alejandra Perina
    Ana M. González-Tizón
    Zeltia Torrecilla
    Andrés Martínez-Lage
    Scientific Reports, 8
  • [27] Development and characterization of 15 polymorphic microsatellite loci in Sonneratia alba (Lythraceae) using next-generation sequencing
    Yoshimi Shinmura
    Alison K. S. Wee
    Koji Takayama
    Takeshi Asakawa
    Orlex Baylen Yllano
    Severino G. Salmo
    Erwin Riyanto Ardli
    Nguyen Xuan Tung
    Norhaslinda Binti Malekal
    Sankararamasubramanian Halasya Onrizal
    Sarawood Meenakshisundaram
    M. Nazre Sungkaew
    Bayu Saleh
    Khin Khin Adjie
    Emiko Soe
    Noriaki Oguri
    Yasuyuki Murakami
    Shigeyuki Watano
    Edward L. Baba
    Tadashi Webb
    Conservation Genetics Resources, 2012, 4 : 811 - 814
  • [28] Development and characterization of 15 polymorphic microsatellite loci in Sonneratia alba (Lythraceae) using next-generation sequencing
    Shinmura, Yoshimi
    Wee, Alison K. S.
    Takayama, Koji
    Asakawa, Takeshi
    Baylen Yllano, Orlex
    Salmo, Severino G., III
    Ardli, Erwin Riyanto
    Nguyen Xuan Tung
    Malekal, Norhaslinda Binti
    Onrizal
    Meenakshisundaram, Sankararamasubramanian Halasya
    Sungkaew, Sarawood
    Saleh, M. Nazre
    Adjie, Bayu
    Soe, Khin Khin
    Oguri, Emiko
    Murakami, Noriaki
    Watano, Yasuyuki
    Baba, Shigeyuki
    Webb, Edward L.
    Kajita, Tadashi
    CONSERVATION GENETICS RESOURCES, 2012, 4 (03) : 811 - 814
  • [29] Isolation and characterization of twenty-six, polymorphic microsatellite loci for the tropical sea urchin, Colobocentrotus atratus
    Benjamin J. Wainwright
    Irma S. Arlyza
    Stephen A. Karl
    Conservation Genetics Resources, 2013, 5 : 379 - 381
  • [30] Isolation and characterization of twenty-six, polymorphic microsatellite loci for the tropical sea urchin, Colobocentrotus atratus
    Wainwright, Benjamin J.
    Arlyza, Irma S.
    Karl, Stephen A.
    CONSERVATION GENETICS RESOURCES, 2013, 5 (02) : 379 - 381
12345