Isolation and characterization of twenty-six, polymorphic microsatellite loci for the tropical sea urchin, Colobocentrotus atratus

被引：0
|
作者：
Benjamin J. Wainwright
Irma S. Arlyza
Stephen A. Karl
机构：
[1] University of Hawai‘i,Hawai‘i Institute of Marine Biology
[2] Mānoa,Research Center for Oceanography
[3] Indonesian Institute of Sciences,undefined
来源：
Conservation Genetics Resources | 2013年 / 5卷
关键词：
Connectivity; Gene flow; Indonesia; Conservation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Twenty-six, polymorphic microsatellite loci were developed for the tropical sea urchin Colobocentrotus atratus. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Roche 454 technology. The 26 loci were characterized in 21 individuals from Pulau Asu, Nias, Indonesia. Observed levels of heterozygosity ranged from 0.52 to 1.00 with a mean of 11.9 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and 3 loci significantly deviated from Hardy–Weinberg expectations.
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页码：379 / 381
页数：2
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